[article]
| Titre : |
1-butyl-3-methylimidazolium acetate as an alternative solvent for type I collagen |
| Type de document : |
texte imprimé |
| Auteurs : |
Jie Liu, Auteur ; Zhou Xu, Auteur ; Chen Yi, Auteur ; Haojun Fan, Auteur ; Bi Shi, Auteur |
| Année de publication : |
2014 |
| Article en page(s) : |
p. 189-196 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Chimie analytique
Collagène -- Solubilité
Solvants -- Suppression ou remplacement
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Low solubility and undesirable denaturation in conventional solvents continue to represent a significant challenge for efficient extraction, accurate characterization and versatile processing of collagen. In the present study, a room temperature ionic liquid (IL), 1-butyl-3-methylimidazolium acetate ([BMIM]Ac), was synthesized, and then evaluated as an alternative solvent for type I collagen. Real-time polarizing optical microscope observation indicated complete disintegration of hierarchical structure of collagen aggregates as solubilized in [BMIM]Ac at 25°C. The solubility reached up to approximately 8.0 wt.% at 25°C, more than ten times higher than that in conventional dilute acetic acid. In comparison with dilute acetic acid and recently reported chloridion ILs, high solubility of collagen in [BMIM]Ac at room temperature was ascribed to loose binding between [BMIM]+ and acetate, as well as stronger proton-accepting ability of [BMIM]Ac, which enabled rupture of those intermolecular hydrogen bonds and ionic bonds that stabilized collagen aggregates. However, such bond-rupturing effect was found selective at room temperature. As demonstrated by Fourier transform infrared, circular dichroism, atomic force microscope, and ultrasensitive differential scanning calorimetry analysis, [BMIM]Ac did not destroy the special triple-helical structure of tropocollagen molecules that had been identified as being of importance for the functional and bioactive properties of collagen. According to these results, the discovery of [BMIM]Ac as an ideal solvent for collagen may open up new possibilities for the chemistry and engineering of collagen, which has long been established as a readily accessible and renewable resource with many unique properties. |
| Note de contenu : |
- EXPERIMENTAL : Materials - Synthesis of [BMIM]Ac - Dissolution of collagen in [BMIM]Ac - Polarizing optical microscope (POM) observation - FTIR analysis - CD measurement - AFM observation - DSC measurement
- RESULTS AND DISCUSSION : Dissolution mechanism of collagen in [BMIM]Ac - Triple-helical structure of collagen regenerated from [BMIM]Ac |
| En ligne : |
https://drive.google.com/file/d/1sm3cYDSvhjqbYBv8WJIDxo4nbF5k-kaO/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=21517 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIX, N° 6 (06/2014) . - p. 189-196
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