Novel double staining of the stratum corneum with fluorescent ε-poly-l-lysine and anionic dextran / Moemi Yoshida in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 3 (06/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 3 (06/2023) . - p. 400-412 Titre : Novel double staining of the stratum corneum with fluorescent ε-poly-l-lysine and anionic dextran Type de document : document électronique Auteurs : Moemi Yoshida, Auteur ; Sana Numajiri, Auteur ; Nao Notani, Auteur ; Koji Nomoto, Auteur ; Hiroya Urabe, Auteur ; Jun-ichi Sato, Auteur ; Yuji Yamashita, Auteur ; Tetsuji Hirao, Auteur Année de publication : 2023 Article en page(s) : p. 400-412 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Barrière cutanée Couche cornée Dermatologie Peau -- Coloration Peau -- Physiologie Polydextrane Polymères cationiques Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : ε-Poly-l-lysine (PLL) is a cationic polymer consisting of 25–35 l-lysine residues. Our previous study revealed that fluorescently labelled PLL can stain the stratum corneum (SC) via ionic interactions between PLL and SC constituents. In this study, to further clarify the mechanisms underlying the interaction between PLL and the SC, the staining properties of fluorescent PLL were compared with that of fluorescently labelled anionic dextran (aDex), which has approximately the same molecular weight as PLL. - Methods : SC samples were collected by non-invasive tape stripping and stained with fluorescent PLL and/or fluorescent aDex. Fluorescence images were acquired using a fluorescence microscope and then analysed. - Results : The SC could be stained with either fluorescent PLL or aDex, both of which were inhibited by the addition of high concentrations of salt solutions. In particular, aDex staining was inhibited at a lower salt concentration than PLL staining. Moreover, PLL staining was inhibited under acidic conditions, while aDex staining was inhibited under neutral to alkaline conditions. Double staining of SC with both fluorescent polymers produced heterogeneous staining patterns: corneocytes stained with both polymers, corneocytes stained with PLL or aDex in a mutually exclusive manner, and unstained corneocytes. Staining of SC samples from the face was more extensive than staining of SC samples from the inside of the upper arm with both polymers. In addition, pretreatment of the SC with ethanol resulted in enhanced staining with both polymers. These results suggest that double staining of SC with both polymers can provide information on the damaged SC. - Conclusion : Staining of SC with fluorescent PLL depends on its properties of a cationic and hydrophobic polymer with appropriate molecular size, which can distinguish the damaged SC. Double staining of SC with fluorescent PLL and aDex is a novel approach to obtain information for the analysis of skin conditions. Note de contenu : - MATERIALS AND METHODS : Reagents - Fluorescent labelling of PLL - Sampling of stratum corneum - Staining of SC with fluorescent polymers - Double staining of SC with fluorescent PLL and aDex - Staining of albumin-adsorbed membrane - RESULTS : Dose dependence of SC staining with fluorescent PLL and aDex - pH dependence of SC staining with fluorescent PLL and aDex - Inhibition of SC staining at high salt concentrations - Regional difference in SC staining with fluorescent PLL and aDex - Double staining of SC with fluorescent PLL and aDex - Characterization of PLL and aDex adsorption by BSA - Perturbation of keratin organization and fluorescent polymer staining DOI : https://doi.org/10.1111/ics.12847 En ligne : https://drive.google.com/file/d/1fsBzMjeEPFgi8e8FI0g0uedzgNxUnSrf/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39570 [article]

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