[article]
| Titre : |
Effect of chrominium in blue hides on activity of microbial transglutaminase |
| Type de document : |
texte imprimé |
| Auteurs : |
Maryann M. Taylor, Auteur ; J. Lee, Auteur ; Lorelei P. Bumanlag, Auteur ; Eduard Hernà ndez Balada, Auteur ; Eleanor M. Brown, Auteur |
| Année de publication : |
2010 |
| Article en page(s) : |
p. 175-180 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Microbial transglutaminase (mTgase) has been used in the modification of proteins from sustainable resources to make products that have application in leather production. It has also been successfully used to treat blue stock, prior to addition of the products, for the purpose of stabilizing the fillers and lessening the chance that they will be washed out during further processing steps. There is always a concern that metals may have an effect (either positively or negatively) on the enzyme's activity and literature has addressed many of these issues. However, one area that has not been addressed is whether chromium may affect activity of mTgase. Even though we have only seen positive results after hide treatment with enzyme, we designed an experiment to elucidate this matter. Using Atomic Absorption Spectroscopy (AAS), we first determined the concentration of chromium in the floats during the processing of the hides. When the hides were washed and then neutralized, the concentration of unbound chromium was found to be less than 5 ppm. Next, to test mTgase sensitivity, we carried out activity assays in absence and presence of chromium and found that activity results were similar. We prepared a model system in which gelatin, swollen and solubilized in solutions containing chromium, was reacted with mTgase using standard conditions. It was found, when compared to a control, that the reaction proceeded at the same rate (chemical gel formed in 2 h @ 50°C), and the physical properties and molecular weight distributions (SDS-PAGE) of the products were similar. Finally, epi-fluorescent microscopy studies showed that enzyme-pretreatment, when compared to a control in which no enzyme pretreatment was applied, prevented migration of the fillers during subsequent processing steps. Thus, under the conditions in which we treat wet blue hides, the chromium used to tan the hides does not affect enzyme activity |
| Note de contenu : |
EXPERIMENTAL : MATERIALS
METHODS : Treatment of blue stock for chromium analyses of floats - Treatment of gelatin with enzyme - Treatment of blue stock for epi fluorescent microscopy studies.
ANALYSES : Measurement of mTgase activity - Physical properties, molecular weight distribution and chromium analysis - Epi-fluorescent microscopy.
RESULTS AND DISCUSSION : Chromium analyses of treatment floats - Reaction of gelatin with mTgase in presence of chromium - Epi-fluorescent microscopy studies of treated blue stock. |
| En ligne : |
https://drive.google.com/file/d/1QPR2TZzLFNQkDcYtt-XjGCN0CEa8sy65/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=9452 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CV, N° 6 (06/2010) . - p. 175-180
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Effect of chrominium in blue hides on activity of microbial transglutaminase in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CV, N° 6 (06/2010)
