| Titre : |
The bioindicator method for assessing safety in use of leathers, Part II : The study of the response of tetrahymena to leather extracts by using a microscope and the Clark electrode |
| Type de document : |
texte imprimé |
| Auteurs : |
Gabriela Zielinska, Auteur ; Ewa Marcinkowska, Auteur |
| Année de publication : |
2016 |
| Article en page(s) : |
p. 327-333 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Cuirs et peaux -- Analyse
Echantillonnage
Electrodes
Indicateurs biologiques
Microorganismes -- Respiration
Microscopie
Tetrahymena pyriformis
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
It has been assumed that the results of calorimetric measurements of heat emissions from the bioindicator cells in extracts taken from leathers (Part 1) are to be confirmed by microscopic studies and measurements of oxygen concentrations related to cellular respiration of Tetrahymena pyriformis. It has been observed that the exposure of the bioindicator in the calorimeter to a harmful extract caused behavioural, morphological and cytophysiological changes in cells. A reduced proliferation rate was also found. The kinetics of the oxygen concentration changes in samples containing the bioindicator and an extract was different from that of samples containing the bioindicator and water. Statistically significant differences between the average oxygen concentration in the systems were compared, thus indicating anomalies in respiratory processes. These results confirm that the changes in heat power values recorded in calorimetric measurements resulted from an effect of extracts on a living organism are related to disturbances in vital functions in cells. |
| Note de contenu : |
- MATERIALS AND METHODS : Microscopic observations - Oxygen concentration measurements
- TEST RESULTS AND ANALYSIS : Microscopic observations of bioindicator cells in water and extracts |
| En ligne : |
https://drive.google.com/file/d/1Me8ElPWPqRFoUk__w8g-Pnbssf1hmh5q/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27669 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 100, N° 6 (11-12/2016) . - p. 327-333
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