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Method to extract water-soluble collagen from induced biphasic commercial gel / Matthew J. Mastauskas in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVII, N° 7 (07/2012)
[article]
inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVII, N° 7 (07/2012) . - p. 243-248
Titre : Method to extract water-soluble collagen from induced biphasic commercial gel Type de document : texte imprimé Auteurs : Matthew J. Mastauskas, Auteur ; Gennaro J. Maffia, Auteur Année de publication : 2012 Article en page(s) : p. 243-248 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Collagène -- Solubilité
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Extraction (chimie)
Gel de collagèneIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Water-soluble collagen extracted from commercial collagen gel could be a possible replacement for water-insoluble collagen fibrils, which are currently extracted from raw corium. If the water-soluble collagen proves to be a suitable replacement in the various water-insoluble collagen applications as mention in this paper, then the time-intensive milling process could be avoided. The first part of the process as described in this paper is a method to extract the water-soluble collagen. Water-soluble collagen was extracted from commercial collagen gel by mixing distilled water with the collagen gel, centrifugation, decanting, freezing and vacuum drying the supernate. Various ratios of commercial collagen gel to water ranging from 10% to 90% commercial collagen gel to water were examined based upon the extraction and performance of gravimetric analyses on the water-soluble collagen. The commercial collagen gel to water ratio had an effect on the mass fraction of extracted water-soluble collagen. Greater amounts of water-soluble collagen could be extracted by using lesser amounts of commercial collagen gel. The water-soluble collagen matrices had a mesh-like appearance similar to cotton, except that they could be flattened easily and could not retain their original shape. The water-soluble collagen matrices were formed into a chaotic, non-uniform manner caused by the re-constituting of fibers during the freezing and vacuum drying process. Note de contenu : - INTRODUCTION : Introduction to collagen - Water-soluble collagen
- EXPERIMENTAL : Experimental overview - Materials - Centrifugation study - Extraction process
- RESULTS AND DISCUSSION : Collagen composition of commercial collagen gel - Mass fraction of water-soluble collagen matrices - Microscopy of commercial collagen gel - Microscopy of water-soluble collagen matrix - SEM - waterxoluble collagen matrix
- CONCLUSIONS : Collagen composition of commercial collagen gel - Mass fraction of water-soluble collagen matrices - Microscopy of commercial collagen gel - Microscopy of water-soluble collagen matrices - SEM - water-soluble collagen matricesEn ligne : https://drive.google.com/file/d/1FwN0_eWMG-lD3IVo86Y7ubbXTn2Wvcz4/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=15644 [article]Réservation
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