Inhibition of propionibacterium acnes lipase by extracts of indian medicinal plants / V. Patil in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 34, N° 3 (06/2012)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 34, N° 3 (06/2012) . - p. 234-239 Titre : Inhibition of propionibacterium acnes lipase by extracts of indian medicinal plants Type de document : texte imprimé Auteurs : V. Patil, Auteur ; A. Bandivadekar, Auteur ; D. Debjani, Auteur Année de publication : 2012 Article en page(s) : p. 234-239 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Acné L'acné (nom féminin) est une dermatose (maladie de la peau) inflammatoire des follicules pilosébacés (glandes sécrétant le sébum, à la racine des poils) avec formation de comédons. Cette maladie de la peau est représentée dans sa forme la plus fréquente par l'acné polymorphe juvénile qui touche les adolescents, mais qui ne résume pas toutes les acnés. Il existe aussi des acnés exogènes d'origine professionnelle, médicamenteuse, cosmétique... Bactéries propioniques Concentration minimale bactéricide Extraits de plantes:Extraits (pharmacie) Peau -- Inflammation Index. décimale : 668.5 Parfums et cosmétiques Résumé : Lipases play an important role in pathogenesis of acne by hydrolysing sebum triglycerides and releasing irritating free fatty acids in the pilosebaceous follicles. Lipase is a strong chemotactic and proinflammatory antigen. Therefore, lipase has generated a high interest as a pharmacological target for antiacne drugs. The aim of this study was to identify inhibitory effects of plant extracts on the lipase activity of Propionibacterium acnes. Colorimetric microassay was used to determine lipase activity. Extracts from Terminalia chebula and Embelia ribes showed lower IC50 value (1 μg mL−1) for lipase inhibition as compared to Vitex negundo and Picrorhiza kurroa (19 and 47 μg mL−1, respectively). The active component responsible for lipase inhibition was isolated. This study reports for the first time the novel antilipase activity of chebulagic acid (IC50: 60 μmol L−1) with minimum inhibitory concentration value of 12.5 μg mL−1 against P. acnes. The inhibitory potential of plant extracts was further confirmed by plate assay. The organism was grown in the presence of subinhibitory concentrations of extracts from P. kurroa, V. negundo, T. chebula, E. ribes and antibiotics such as clindamycin and tetracycline. Extract from T. chebula showed significant inhibition of lipase activity and number of P. acnes. Note de contenu : - MATERIALS AND METHODS : Plant material - Reagents and chemicals - Evaluation of antimicrobial activity by cup plate diffusion method - Determination of minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) values - Evaluation of lipase activity by colorimetric microassay - Determination of antilipase activity of plant extracts by plate assay method - Determinationof viable count by spread plate method - Extraction of chebulagic acid by preparative high performance thin layer chromatography (HPTLC) - RESULTS : Antipropionibacterium assay by agar cup diffusion, MIC and MBC - Isolation of chebulagic acid - Inhibition of lipase activity DOI : 10.1111/j.1468-2494.2012.00706.x En ligne : http://onlinelibrary.wiley.com/doi/10.1111/j.1468-2494.2012.00706.x Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=15033 [article]

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Minimal bactericidal concentration for a quaternary ammonium compound used in soak liquors / Canan Veyselova in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 97, N° 4 (07-08/2013)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 97, N° 4 (07-08/2013) . - p. 166-171 Titre : Minimal bactericidal concentration for a quaternary ammonium compound used in soak liquors Type de document : texte imprimé Auteurs : Canan Veyselova, Auteur ; Meral Birbir, Auteur ; Didem Berber, Auteur Année de publication : 2013 Article en page(s) : p. 166-171 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Ammoniums quaternaires Antibactériens Concentration minimale bactéricide Peaux brutes -- Trempe Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The soaking process is an ideal environment for bacterial growth and bacterial activity during this process causes severe damages on hides. The use of an effective bactericidal agent during soaking is very important to prevent the detrimental effect of bacteria on hides. A quaternary ammonium compound (QAC) containing 12.5% didecyl dimethyl ammonium chloride and 12.5% benzyl dimethyl ammonium chloride which is used in soaking liquors in Turkiye was examined for its effect on Bacillus licheniformis, Bacillus pumilus, Staphylococcus intermedius, Pseudomonas luteola, Enterobacter cloacae, Vibrio fluvialis and Enterococcus faecium. Staphylococcus intermedius, Pseudomonas luteola, Enterobacter cloacae and Enterococcus faecium were isolated from soak liquor containing 0.8g/L of the test agent. Bacillus licheniformis, Bacillus pumilus and Vibrio fluvialis were isolated from salt-pack-cured hides. These bacteria were commonly found in isolates from salted hides. Thé effect of the test agent at different concentrations on the bacteria was examined by the Kirby-Bauer disk diffusion method on Mueller Hinton Agar both at 25°C and at 37°C. In addition, the Minimal Bactericidal Concentration of the agent for the bacteria and the mixed population of these bacteria was also determined in Mueller Hinton Broth at 37°C. All concentrations of the agent produced an inhibition zone for all of the test bacteria. Inhibition zone diameter changed according to concentration of agent and the species of bacteria. The agent was found to be more effective at 25°C than at 37°C. 2.97g/L of the agent in Mueller Hinton Broth was found to kill both the bacteria and mixed culture at exposure times of both 8 and 24 hours at 37°C. It was concluded that the agent should be used in higher concentrations in soak liquors to inactivate the bacteria that might cause major damages on hides. Note de contenu : - INTRODUCTION : The range of products employed - Resistance to antibacterials - Categories of agent - Previous study - EXPERIMENTAL PROCEDURES : Test bacteria and agent - Determination of protease activity - Determination of lipase activity - Assessment of the efficiency of the test agent on the test bacteria at different concentrations at 25°C and at 37°C by Kirby-Bauer disc diffusion method - Determination of the Minimal Bactericidal Concentration of the test agent on the test bacteria and their mixed culture at 37°C - Bacteria isolated from soak liquor treated with QAC - Bacteria previously found on salt-pack-cured hides - Proteases and lipases found in this study - Previous studies - Results of present study En ligne : https://drive.google.com/file/d/12n4-3uVqGRrYZLcklkvihC_-s-X855_H/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=19221 [article]

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De-oiled karanja cake as potential bio-aditive for low salt raw skin preservation / V. Brinda in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXV, N° 5 (05/2020)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXV, N° 5 (05/2020) . - p. 159-165 Titre : De-oiled karanja cake as potential bio-aditive for low salt raw skin preservation Type de document : texte imprimé Auteurs : V. Brinda, Auteur ; N. Vedaraman, Auteur ; Victor John Sundar, Auteur ; J. Kangaraj, Auteur ; K. C. Velappan, Auteur ; Chellappa Muralidharan, Auteur Année de publication : 2020 Article en page(s) : p. 159-165 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Antibactériens Bains de trempe -- Analyse Chlorure de sodium -- Suppression ou remplacement Chromatographie en phase liquide à hautes performances Concentration minimale bactéricide Concentration minimale inhibitrice (antimicrobiens) En microbiologie, la concentration minimale inhibitrice (CMI) est la plus faible concentration d'un produit chimique, généralement un médicament, qui empêche la croissance visible d'une ou de plusieurs bactéries. La CMI dépend du micro-organisme considéré, de l'être humain affecté (in vivo uniquement) et de l'antibiotique lui-même. La CMI est déterminée en préparant des solutions du produit chimique in vitro à diverses concentrations croissantes, en incubant les solutions avec des groupes séparés de bactéries en culture et en mesurant les résultats en utilisant une méthode de dilution standardisée (agar ou microdilution). Les résultats se classent ensuite comme "sensible", "intermédiaire" ou "résistant" à un antimicrobien particulier en utilisant un point d'arrêt. Les points d'arrêt sont des valeurs convenues, publiées dans les directives d'un organisme de référence, comme le US Clinical and Laboratory Standards Institute (CLSI), la British Society for Antimicrobial Chemotherapy (BSAC) ou le Comité européen sur les tests de sensibilité aux antimicrobiens (EUCAST). On a pu constater des écarts importants au niveau des points d'arrêt de divers pays européens au fil des ans, et entre ceux de l'EUCAST et du CLSI. Alors que la CMI est la concentration la plus faible d'un agent antibactérien nécessaire pour inhiber la croissance visible, la concentration bactéricide minimale (CBM) est la concentration minimale d'un agent antibactérien qui entraîne la mort bactérienne. Plus la CMI est proche de la CBM, plus le composé est bactéricide. La première étape de la découverte d'un médicament est souvent le dépistage d'un médicament candidat de banque de données pour les CMI contre les bactéries d'intérêt. En tant que tels, les CMI sont généralement le point de départ pour de plus grandes évaluations précliniques de nouveaux agents antimicrobiens. Le but de la mesure de la concentration minimale inhibitrice est de s'assurer que les antibiotiques sont choisis efficacement pour augmenter le succès du traitement. (Wikipedia) Cuirs et peaux -- Conservation Cuirs et peaux -- Teneur en eau Huile de karanja et constituants Industrie -- Pollution -- Lutte contre Microscopie électronique à balayage Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The most commonly practiced preservation processes for raw hide/skin is by application of common salt (NaCl) due to its wide availability, cost effectiveness and bacteriostatic activity. During the soaking process, the salt present in the skin dissolves in water, thereby generating high total dissolved solids (TDS) levels causing pollution to the environment. Hence, an eco–friendly material without salt or with lower quantities of salt is needed for the preservation of the skin. In this present study Karanja de-oiled cake (Millettia/Pongamia pinnata) extract was studied for its antibacterial activity, minimal inhibitory concentration and minimum bactericidal concentration for preservation. The reverse phase high performance liquid chromatography (RP-HPLC) analysis shows the presence of karanjin (0.2%) and pongamol (0.02%) and these compounds may be responsible for the antibacterial activity against skin deteriorating bacteria. The preservation trials with karanja de-oiled cake 15% along with less salt 15% showed no hair slip, no putrefaction, reduced bacterial load to approximately 35%, compared to control skins at 25º-35ºC for 21 days. The preserved skins on rehydration showed that the soak liquor of experimental skins had a considerable reduction in TDS (about 67%) compared with conventional soak liquor. On processing these skins into leather, the experimental skins were found to possess comparable strength properties (tensile, tear strength) and organoleptic properties as conventional salt-based preserved skins. This study concludes that conventional salt-based preservation of skins can be replaced by an eco-friendly preservation method using karanja de-oiled cake along with less salt. Note de contenu : - MATERIAL AND METHOD : Chemicals - Skin - De-oiled karanja extracts - Isolation and identification of proteolytic bacteria - Antibacterial activity - Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Count (MBC) - HPLC - PRESERVATION EXPERIMENTS : Preliminary studies - Lab scale studies - Moisture content - Bacterial load - SEM analysis - Investigation for pollution load in soak liquor - Leather processing - RESULTS AND DISCUSSION : Physicochemical characterization of leather DOI : https://doi.org/10.34314/jalca.v115i5.3807 En ligne : https://drive.google.com/file/d/1uVYysIEYThstbXhpavFHk1-saEltSUWg/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34161 [article]

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