[article]
| Titre : |
The effect of trypsin on soaking of salt cured hides |
| Type de document : |
texte imprimé |
| Auteurs : |
Luis A. Zugno, Auteur |
| Année de publication : |
1992 |
| Article en page(s) : |
p. 207-220 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Trypsin was biotinylated with NHS-biotin(N-hydroxysuccinimido biotin) and standard cattlehide soaking experiments were conducted in a TRIS buffer solution at pH 7.8 containing 0.02M of calcium ion. The experiments were performed in an equipment that provided temperature control and mechanical agitation; the flesh and grain sides of the hide were submitted to soaking,individually.The soaking time was 4 hours at 30°C.
The biotinylated trypsin was detected inside the hide by the biotin-avidin peroxidase technique, and an insoluble dark brown polymer was formed after the oxidation of DAB(3,3'-diaminobenzidine).With the concentrations and experimental conditions used trypsin had limited penetration in the grain and flesh side and increased penetration in highly area.In the grain, epidermal-dermal separation was observed.Permeability and enzyme penetration increased by increasing the enzyme concentration,suggesting that better soaking and unhairing is obtained.Pieces treated with trypson in the grain produced leather with cleaner grain than was shown by the control. |
| En ligne : |
https://drive.google.com/file/d/1DLAgUELv9ButhKKov1ItJuaNOjFgfpjS/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=8288 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > LXXXVII, N° 6 (06/1992) . - p. 207-220
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The effect of trypsin on soaking of salt cured hides in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), LXXXVII, N° 6 (06/1992)
