| Titre : |
Novel procedure for large-scale purification of atelocollagen by selective precipitation |
| Type de document : |
texte imprimé |
| Auteurs : |
S. S. Maier, Auteur ; I. Buciscanu, Auteur ; V. Maier, Auteur |
| Année de publication : |
2010 |
| Article en page(s) : |
p. 1-8 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Collagène
Précipitation (chimie)
Produits chimiques -- Purification
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
This paper describes a novel procedure for the purification of raw atelocollagen solutions, based on a multi-stage selective precipitation of the protein fractions, using polyethylene glyclos (PEGs) as crowding agents. The precipitation selectivity was provided by the simultaneous effect of pH and crowding agents. Two adjuvants were added to improve the precipitation selectivity : ethanol and trimethylamine-N-oxide (TMAO). An optimal composition of the crowding mixture, consisting of 23 % PEG 400, 61 % PEG 6000 and 16 % PEG 20000, induced a "screening" effect within the isoelectric range of atelocollagen. The optimal recipe for the screening of an atelocollagen sol obtained at plant scale was : 80g/L crowding mixture, 0.2 molar TMAO, 0.3 molar ethanol, pH = 3.8, processing time : 4-6 hr at 5 / 10°C. The proposed procedure is useful in the manufacture of colloidal collagen solutions, in order to replace or to facilitata expensive and denaturing operations, like ultrafiltration. |
| Note de contenu : |
- Materials and reagents
- Methods
- The primary atelocollagen solution
- The enzyme refining
- The ultrapurification procedure
- The colloidal system turbidity
- Total protein nitrogen content
- Undesirable protein fractions and debris, pp
- Table 1 : The restricted mixture design matrix and the measured responses values
- Table 2 : Coefficients of the mathematical models fitted to mixture formulation experimental data |
| En ligne : |
https://drive.google.com/file/d/1k0xMjjuCPWzGJFU8lgIJ0GGViIub8c_x/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=8083 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CV, N° 1 (01/2010) . - p. 1-8
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Novel procedure for large-scale purification of atelocollagen by selective precipitation in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CV, N° 1 (01/2010)
