| Titre : |
Molecular size and conformation of protein recovered from chrome shavings |
| Type de document : |
texte imprimé |
| Auteurs : |
Eleanor M. Brown, Auteur ; C. J. Thompson, Auteur ; Maryann M. Taylor, Auteur |
| Année de publication : |
1994 |
| Article en page(s) : |
p. 215-220 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Partially hydrolyzed collagen is one product of the treatment for chrome shavings developed in this laboratory. The value of this process to the tanner depends, in part, on the market value of this proteinaceous material. Modification of the process at any of several points may alter the characteristics of the protein product and thus improve its market potential. The molecular size of the collagen fragments and thermal stability of collagen-like conformations are both factors in determining possible uses for this material. Polyacrylamide gel electrophoresis SDS-PAGE) was used to analyse the protein fragments. These fragments were extracted from chrome shavings with NaOH, KOH or the respective carbonates. Results of these analyses showed a wide dispersion of molecular weights in all of the samples with the heaviest concentrations in the 100-200 kDa and 50-100 kDa fractions. About 25% of the protein was degraded into fragments ≤50 kDa and a smaller portion was in the form of aggregates ≥200 kDa. When aqueous solutions were held at 5°C for at least 15 hrs, these protein fractions developed 30-60% of the triple helical conformation of a comparable concentration of native collagen as measured by circular dichroism. All fractions extracted with MgO/carbonate mixtures developed more than 50% helical structure while those extracted with MgO/hydroxyde mixtures developed 30-50% helix. Melting points for the helix to coil transition were 22°C ± 2°C for all samples studied. |
| En ligne : |
https://drive.google.com/file/d/1jwICyeh11Sk0iMxfCDVhdp9i9iphMtY3/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=8061 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXIX, N° 7 (07/1994) . - p. 215-220
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Molecular size and conformation of protein recovered from chrome shavings in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. LXXXIX, N° 7 (07/1994)
