| Titre : |
Olive leaf-derived PPAR agonist complex induces collagen IV synthesis in human skin models |
| Type de document : |
document électronique |
| Auteurs : |
George P. Majewski, Auteur ; Smrita Singh, Auteur ; Krzysztof Bojanowski, Auteur |
| Année de publication : |
2021 |
| Article en page(s) : |
p. 662-676 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Antiâge
Barrière cutanée
Cellules -- Cultures et milieux de culture
Collagène
Collagène -- Synthèse
Dermo-cosmétologie
Extraits de plantes:Extraits (pharmacie)
Modèles numériques
Olivier et constituants
Peau -- Soins et hygiène
Peau humaine
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Introduction : Peroxisome proliferator-activated receptor (PPAR) agonists are known to modulate the synthesis of dermal lipids and proteins including collagens. Olive (Olea europaea) leaves have been reported to contain PPAR-binding ligands. Collagen IV, a major dermal-epidermal junction (DEJ) protein, degrades with both age and disease. Here, we report the formulation of a novel multi-ligand complex, Linefade, and its effects on collagen IV synthesis.
- Methods : Linefade prepared from the leaves of Olea europaea contains 2% w/w plant extract solids dissolved in a mixture of glyceryl monoricinoleate and dimethyl isosorbide. In silico docking was performed with PPAR-α (PDB ID: 2P54). Linefade was evaluated for PPAR-α-dependent transcription in a luciferase reporter assay system. Cell viability and collagen IV levels in human dermal fibroblast cultures were measured using the MTT method and ELISA assay, respectively. Transcriptome analysis was conducted on a full-thickness reconstituted human skin (EpiDermFT) model. Ex vivo cell viability and collagen IV immunostaining were performed on human skin explants.
- Results : In silico docking model of the major constituents (oleanolic acid and glyceryl monoricinoleate) produced a co-binding affinity of −6.7 Kcal/mole. Linefade significantly increased PPAR-α transcriptional activity in CHO cells and collagen IV synthesis in adult human dermal fibroblasts. Transcriptome analysis revealed that 1% Linefade modulated the expression of 280 genes with some related to epidermal differentiation, DEJ, PPAR, Nrf2 and retinoid pathways. An ex vivo human explant study showed that 1% Linefade, delivered via a triglycerides excipient, increased collagen IV levels along the dermal–epidermal junction by 52%.
- Conclusion : In silico modelling and in vitro and ex vivo analyses confirmed Linefade-mediated activation of PPAR-α and stimulation of collagen IV synthesis. |
| Note de contenu : |
MATERIALS AND METHODS : Materials - Preparation of linefade - In silico modelling technique and validation - In vitro cell studies - Assay on reconstituted skin substitutes - Ex vivo study - Statistical analysis
- RESULTS : In silico modelling - In vitro cell studies - Transcriptome analysis - Human explants (Collagen IV) |
| DOI : |
https://doi.org/10.1111/ics.12742 |
| En ligne : |
https://drive.google.com/file/d/1BeDt-gYbDKZbvjDvfvDYue6axRMi-oNy/view?usp=shari [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37048 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 6 (12/2021) . - p. 662-676
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