| Titre : |
Measurement of collagenase activity in the tannery and in the laboratory I. the assay medium |
| Type de document : |
texte imprimé |
| Auteurs : |
S. M. Mozersky, Auteur ; David G. Bailey, Auteur |
| Année de publication : |
1995 |
| Article en page(s) : |
p. 357-364 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675.2 Préparation du cuir naturel. Tannage |
| Résumé : |
A commercially available acidic solution of pepsin-treated porcine skin collagen designed for cosmetic use was shown to be suitable as a substrate for viscometric assay of collagenase activity when adjusted to near neutrality and diluted with the proper buffer to a collagen concentration of 1.5 to 4.5 g/L. The buffer contains tris(hydroxymethylamino) methane (tris), citrate, and calcium (ca2+). The pH of the assay solution is 6.8. The assay solution can be supplemented with NaCl up to a concentration of at least 0.4M and the pH can be raised to at least 8.5. Pepsin treatement of calfskin colagen yields a product whose viscometric behavior in the presence of collagenase is virtually identical to that of the commercial porcine product. |
| En ligne : |
https://drive.google.com/file/d/1-lgi2uHxGd-Ol-t2F10SSzc1LRIUjNCU/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=7861 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXX, N° 12 (12/1995) . - p. 357-364
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Measurement of collagenase activity in the tannery and in the laboratory I. the assay medium in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. LXXXX, N° 12 (12/1995)
