Titre : |
Design of a capillary viscometer for assay or collagenase activity by "continuous" observation of the hydrolysis of a soluble collagen with a time of flight flowmeter |
Type de document : |
texte imprimé |
Auteurs : |
Samuel M. Mozersky, Auteur ; David G. Bailey, Auteur |
Année de publication : |
1996 |
Article en page(s) : |
p. 263-269 |
Note générale : |
Bibliogr. |
Langues : |
Américain (ame) |
Index. décimale : |
675 Technologie du cuir et de la fourrure |
Résumé : |
The design of a capillary viscometer for semi automated assay of collagenase activity is described. The rate of flow of a solution of a soluble derivative of collagen undergoing hydrolysis by the collagenase is measured "continuously" by an electronic flow meter. Since the reciprocal of the flow-rate is proportional to the viscosity and the latter is a (double exponential decay) function of the collagenase concentration (as well as of time), collagenase concentration can be determined from the flowrate at a given incubation time. The data are collected automatically, stored in a computer, and analyzed at the analyst's convenience. The viscometer is suitable for use in research laboratories investigating damage to hides (and therefore to leather) caused by bacterial digestion of collagen. |
En ligne : |
https://drive.google.com/file/d/12KH7lJZFUSBibEbZKbcB8Ya5PA_Vxrbz/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=7788 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXXI, N° 10 (10/1996) . - p. 263-269