[article]
| Titre : |
Viscometric assay of collagenase activity using a stop reagent to terminate enzyme action |
| Type de document : |
texte imprimé |
| Auteurs : |
Samuel M. Mozersky, Auteur ; David G. Bailey, Auteur |
| Année de publication : |
1997 |
| Article en page(s) : |
p. 62-69 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
A simple, precise viscometric assay procedure has been developed for the assay of true collagenase activity. The substrate is a commercially available pepsin-treated mammalian skin collagen. It comes as a solution which needs no treatment other than pH adjustment and dilution prior to its incorporation into the assay medium. Viscosity is measured after enzymatic action is terminated with a Stop Reagent. Any one of a wide variety of viscometers can be used, including a simple Ostwald-type viscometer. Other advantages of the procedure are also discussed. The temperature during incubation and viscometry should be controlled within + or – 0,1°C. Such control is easily achieved with an inexpensive controller. |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=7683 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCII, N° 3 (03-04/1997) . - p. 62-69
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