[article]
| Titre : |
Optimization of the assay of bate and drum liquor for proteolytic activity |
| Type de document : |
texte imprimé |
| Auteurs : |
Samuel M. Mozersky, Auteur ; Marvin A. Baynes, Auteur ; David G. Bailey, Auteur |
| Année de publication : |
1995 |
| Article en page(s) : |
p. 235-242 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
In two recent publications from this laboratory, procedures were described for measuring the proteolytic activity of bate and (bate-containing) drum liquor using hide powder azure (HPA) as substrate. Subsequent to that work, it was found that HPA sometimes loses much of its sensitivity to proteolytic enzymes of storage. This led to a study of the substrate and of related factors affecting the assay, which resulted in substantial revision of the assay procedure, as follows. HPA is activated prior to use by reduction of particle size and a mild heat treatment, and200mg used per assay (instead of 50mg). A clarified extract of the sample to be assayed is introduced into the incubation tube (a 25 mm ´ 150 mm screw-cap tube) instead of the crude material. Since proteolytic activity was found to change by 10 to 15% per °C, good temperature control is necessary; for research purposes, a water-bath shaker-incubator operating at 37°C and 400 rpm is recommended. Incubation time has been reduced from 20 min to 10 min at ambient temperature (ca. 25°C), 5 min at 37°C. These modifications have resulted in the following advantages: (1) substantially increased sensitivity, (2) a linear dependence of the color measured on enzyme concentration, and (3) improved reproducibility. |
| En ligne : |
https://drive.google.com/file/d/1m4ZmYp-cbBTCpJwnoL3XdK7s8DMhbC5P/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=7931 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXX, N° 8 (08/1995) . - p. 235-242
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