| Titre : |
Properties of bovine hair keratins solubilized with thioglycolate |
| Type de document : |
texte imprimé |
| Auteurs : |
A. M. S. Kamal, Auteur ; K. Shirai, Auteur ; Y. Ishii, Auteur ; Y. Nomura, Auteur |
| Année de publication : |
1998 |
| Article en page(s) : |
p. 272-282 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Partially decomposed hair, created during conventional hide processing in the beamhouse, is one of the strongest pollutants in the tannery effluent. One of the approaches that has been taken to reducing the pollution from the unhairing process is to remove intact hair from the hides by using conventional reagents in novel ways that loosen rather than digest the hair. This paper considers the reductive solubilization of recoverde hair from raw hide with the final goal being to utilize it as a bio-material. In the present study, three kinds of solubilizing solutions were tested : 0.1 M thioglycolate at pH 11 35°C (TG-11) , 0.1 M thioglycolate containing 6M guanidine at pH 7 35°C (TG/G-7) and 0.1M thioglyconate containing 6M urea at pH 7 , 35°C (TG/U-7. About 60% of the original hair weight was recovered as the product became solubilized with TG-11. The recovered protein had good solubility in solvent buffer without reducing agent. Approximately the same extent of solubilization was attained with TG/G-7 at neutral pH. The reultant preparation was almost insoluble unless the solvent contained the reducing agent. One the other hand, the extent of solubilization with TG/U-7 was significantly low. |
| Note de contenu : |
- EXPERIMENTAL: Bovine hair - Dissolution of hair and recovery of product - Solubility test - Amino acid analysis - SDS-polyacrylamide gel electrophoresis - Size exclusion chromatography (SEC) - FTIR analysis
- RESULTS AND DISCUSSION: Amino acid composition of different kinds of bovine hairs and merimo wool - Yield of the dissoved product from hair in thioglycolate solution - Solubility of dissolved product in non-reductive solvent buffer - Amino acid composition of dissoved products - SDS-PAGE of dissolved products - SEC pattern of the dissolved product - FTIR analysis of dissolved product |
| En ligne : |
https://drive.google.com/file/d/1nvwS5396h6KE_5GJNjywJ2j6rl6nGmFG/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4496 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCIII, N° 9 (11/1998) . - p. 272-282
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