| Titre : |
Viscometric assay of the protease activity of bate and drum liquor |
| Type de document : |
texte imprimé |
| Auteurs : |
S. M. Mozersky, Auteur ; David G. Bailey, Auteur ; Jill Deiss, Auteur |
| Année de publication : |
1999 |
| Article en page(s) : |
p. 51-58 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
A viscometric procedure has been developed for the assay of general (non-specific) proteolytic activity of bate in the drum during the course of the bating operation. The basic features of the assay procedure are as follows : (1) a commercially available gelatin is used as substrate, (2) a clarified sample of drum liquor is added, (3) the resulting mixture is incubated at 40°C for 20 min or 60 min, (4) enzymatic activity is terminated with a stop reagent, and (5) the viscosity of the resulting solution is measured at 40°C. Any one of variety of instruments may be used to measure the viscosity, including a simple glass viscometer of the Cannon-Fenske type. The dependence of the measured viscosity on the concentration of proteolytic enzyme is well described be a double exponential decay curve. Numerous samples can be run simultaneously (with staggering). |
| Note de contenu : |
- MATERIALS AND METHODS : Equipment - Solids and solutions - Sample preparation - Incubation - Measurement of viscosity
- RESULTS AND DISCUSSION : Time course of gelatin hydrolysis - Dependence of viscosity on enzyme concentration - Preliminary application of the assay procedure - Calculations
|
| En ligne : |
https://drive.google.com/file/d/1mkaYpxB2vZ376iZdkcgooU92nr3Wqu_o/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4488 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCIV, N° 2 (02-03/1999) . - p. 51-58
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