A new approach to less-salt preservation of raw skin/hide / James Kanagaraj in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. XCV, N° 10 (12/2000)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCV, N° 10 (12/2000) . - p. 368-374 Titre : A new approach to less-salt preservation of raw skin/hide Type de document : texte imprimé Auteurs : James Kanagaraj, Auteur ; Chandra Babu Narasimhan Kannan, Auteur ; S. Sadulla, Auteur ; G. Suseela Rajkumar, Auteur ; V. Visalakshi, Auteur ; N. Chandrakumar, Auteur Année de publication : 2000 Article en page(s) : p. 368-374 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Salt curing systems, despite their cost and ease advantages, suffer heavily on environmental counts. The ill effects of the system need no emphasis. The deteriorating effect of electrolytes discharged by the use of salt by the tanning industry on the quality of soil and ground water is realised now more than ever before. The electrolytes, in the form of chloride and sulphate, form the largest component of most tannery effluents and they are the most difficult to treat at the end of the pipe. The source of the electrolytes is mainly from salt curing methods currently followed. The objective of this paper is to propose a suitable technology for the preservation of raw skin/hide that is an alternative to the salt curing method so as to combat the pollution problem arising out of salt. The present work is aimed at developing a less-salt curing method. The entire work is based on the fact that salt curing depends on dehydration of the stock to less than 30% water content in addition to exploiting the bacteriostatic properties of salt. In the present investigation, the preservation effect is achieved by using a combination of silica gel - environmental friendlier and easy-to-treat powerful dehydrating agent and 5% salt with or without 0.1% of p-chloro meta cresol (PCMC). The efficacy of the method was assessed by various parameters and tools such as moisture content, total extractable nitrogen, bacterial count and pollution load generated in leather processing. The results indicate that the method was as efficient as conventional salt curing and did not pose any problem in soaking or in the leather manufacturing process. The quality of the leather obtained was on a par with that obtained from conventionally salt cured stock. Thus the method appears to be viable in combating the problems of chloride and Total Dissolved Solids (TDS) and may be an effective alternative to conventional salt curing . En ligne : https://drive.google.com/file/d/1sQj1QjAuDd6fG82BabL5cITpJm3FO468/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4404 [article]

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Recovery and characterization of protein hydrolysate from chrome shavings by microbial degradation / C. Shanthi in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVIII, N° 6 (06/2013)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVIII, N° 6 (06/2013) . - p. 231-239 Titre : Recovery and characterization of protein hydrolysate from chrome shavings by microbial degradation Type de document : texte imprimé Auteurs : C. Shanthi, Auteur ; Pradipta Banerjee, Auteur ; Chandra Babu Narasimhan Kannan, Auteur ; G. Suseela Rajkumar, Auteur Année de publication : 2013 Article en page(s) : p. 231-239 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries Biodégradation Caractérisation Collagène Cuirs et peaux -- Déchets -- Recyclage Hydrolysats de protéines Récupération (Déchets, etc.) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Chromium containing collagenous solid wastes from the leather tanning industry requires special attention because of the pressure exerted by environmental authorities for safe disposal. Such collagen-rich wastes can be recycled by adapting environmentally suitable and safer methods based on microorganism and/or enzymatic treatment, so as to obtain a collagen hydrolysate with potential applications. In the present study, chromium tolerant bacteria were isolated from tannery soil and screened for high proteolytic activity by zone-clearance assay and caseinolytic activity. The most potent bacterium, with a high proteolytic and chromium-tolerant ability was found to degrade about 90% of the chrome shavings in 120h and was identified as Alcaligenes faecalis. The hydrolysates at various time intervals of proteolysis were collected, chromium removed and characterized. The hydrolysate was found to have 12% ash and 80% protein or peptides contents after 120h of proteolysis with 3.14±2.0?g of chromium/g of protein. Molecular weight profiling done by gel filtration chromatography using sephadex G 25 and tricine-SDS-PAGE electrophoresis revealed that the major component of the hydrolysate comprised of small peptides in the molecular weight range of 3-30kDa. Note de contenu : - MATERIALS AND METHODS : Isolation of organism - Protease activity - Chromium tolerance - Degre of hydrolysis (%) and Cr3 removal from the chrome shavings - Analysis of the protein hydrolysate - pH determination by deionization - Gel filtration chromatography - Ion exchange chromatography - Tricine SDS-PAGE of the hydrolysate - RESULTS AND DISCUSSION : Protease activity, identification and chromium tolerance of the screened bacterium - Chrome shaving hydrolysis process - Characterization of protein hydrolysate - Determination of molecular weight of protein hydrolysate by gel filtration and gel electrophoresis En ligne : https://drive.google.com/file/d/1LQ3kblknV4gm5BdYm3xIrye-aspbynzD/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=18746 [article]

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