A combination of three antioxidants decreases the impact of rural particulate pollution in Normal human keratinocytes / Angelica Ortiz in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 6 (12/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 6 (12/2023) . - p. 791-801 Titre : A combination of three antioxidants decreases the impact of rural particulate pollution in Normal human keratinocytes Type de document : texte imprimé Auteurs : Angelica Ortiz, Auteur ; Hong Sun, Auteur ; Thomas Kluz, Auteur ; Mary S. Matsui, Auteur ; Tiffany Carle, Auteur ; David Gan, Auteur ; Terry Gordon, Auteur Année de publication : 2023 Article en page(s) : p. 791-801 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antioxydants Cosmétiques Dermo-cosmétologie Kératinocytes Les kératinocytes sont des cellules constituant 90 % de la couche superficielle de la peau (épiderme) et des phanères (ongles, cheveux, poils, plumes, écailles). Ils synthétisent la kératine (kératinisation), une protéine fibreuse et insoluble dans l'eau, qui assure à la peau sa propriété d'imperméabilité et de protection extérieure. L'épiderme est divisé en 4 couches basées sur la morphologie des kératinocytes (de l'intérieur vers l'extérieur) : 1. stratum germinativum (couche basale à la jonction avec le derme) 2. stratum spinosum 3. stratum granulosum 4. stratum lucidum 5. stratum corneum Les kératinocytes passent progressivement de la couche basale vers les couches supérieures par différenciation cellulaire jusqu'au stratum corneum ou ils forment une couche de cellules mortes nommées squames, par apoptose. Cette couche constitue une barrière de protection et réduit la perte d'eau de l'organisme. Les kératinocytes sont en perpétuel renouvellement. Ils mettent environ 1 mois pour aller de la couche basale au stratum corneum mais ce processus peut être accéléré en cas d'hyperprolifération de kératinocyte (psoriasis). Particules fines Peau -- Effets de la pollution atmosphérique Peau -- Soins et hygiène Stress oxydatif Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : It is well established that exposure of human skin to airborne pollution, particularly in the form of particulate matter sized 2.5 μm (PM2.5), is associated with oxidative stress, DNA damage and inflammation, leading to premature signs of skin aging. Because much of the damage results from oxidative stress, we examined the effects of a topical composition containing three antioxidants in an in vitro model system to assess the potential for amelioration of premature aging. The use of multiple antioxidants was of interest based on the typical composition of therapeutic skincare products. It is important to determine the efficacy of multiple antioxidants together and develop a short-term assay for larger scale efficacy testing. - Methods : Normal human epidermal keratinocytes were exposed to a rural-derived source of PM2.5 in the presence and absence of an antioxidant mixture of resveratrol, niacinamide and GHK peptide. Endpoints related to inflammation, premature aging and carcinogenicity were monitored after 5 h of exposure and included IL-6, CXCL10, MMP-1 and NRF2. Differentially expressed genes were monitored by RNA-seq. - Results : Pre-treatment of keratinocytes with the antioxidant preparation in the absence of PM2.5 reduced baseline levels of MMP-1, IL-6 and CYP1A1 and reduced PM2.5-induced increases in all four endpoints, MMP-1, IL-6, CXCL10 and CYP1A1. Antioxidants significantly increased NRF2 protein in the presence of PM2.5, indicating a protective response. RNA-seq interrogation of antioxidant-treated cells further showed increased expression of NRF2 inducible genes. The expression of CYP1A1 and genes related to aryl hydrocarbon activation were induced by PM2.5 and suppressed by antioxidants. - Conclusions : Specific signalling pathways known to be correlated with skin inflammation and aging were examined based on their suitability for use in efficacy testing for the prevention of skin damage due to ambient hydrocarbon pollution. Endpoints examined after only 5 h of exposure provide a useful method amenable to high through-put screening. The results obtained reinforce the concept that a multiple antioxidant preparation, topically applied, may reduce pro-inflammatory signalling and cellular damage and thereby reduce premature skin aging due to exposure to rural-derived airborne pollution. Note de contenu : - MATERIALS AND METHODS : Cell culture - Exposure to rural PM Antioxidants - Cell viability - Assessment of oxidative stress, reactive oxygen species - IL-6 and MMP-1 measured by ELISA assays - NRF2 protein expression levels measured by Western blot - CYP1A1 and CXCL10 (IP-10) measured by RT-PCR analysis - RNA sequencing analysis of differentially expressed genes - Data analysis - RESULTS : Determination of optimal noncytotoxic exposure conditions for PM2.5 and AOx - IL-6 protein release by NHEK - Induction of CXCL10 mRNA by PM2.5 and inhibition by AOx in NHEK - NHEK expression of CYP1A1 mRNA - MMP-1 protein release by NHEK - Induction of NRF2 protein - RNA sequencing analysis identified DEGS En ligne : https://drive.google.com/file/d/1CshhBS3Ys2LrFsxeukcuSlS5LYn15BVZ/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40265 [article]

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