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Fungal biotransformation of bovine hair : Part 1 - Isolation of fungus with keratinolytic activity / B. C. Galarza in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 88, N° 3 (05-06/2004)
[article]
Titre : Fungal biotransformation of bovine hair : Part 1 - Isolation of fungus with keratinolytic activity Type de document : texte imprimé Auteurs : B. C. Galarza, Auteur ; L. M. Goya, Auteur ; Carlos S. Cantera, Auteur ; Maria Laura Garro, Auteur ; H. E. Reinos, Auteur ; Laura M. I. Lopez, Auteur Année de publication : 2004 Article en page(s) : p. 93-98 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bovins
Détérioration fongique
Poils -- BiodégradationIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : The present study reports on the results of the “fungal biodegradation” of “hair waste” in a static solid medium using previously isolated strains of Trichophyton ajelloi and Fusarium oxysporum. The main purpose of the research programme is to upgrade the hair by biodegradation, to consider it as a raw material instead of the current modality of “throwing away” proteins to landfill and to its reuse in agricultural technology, directly or after composting and applying earthworms to generate an organic fertilizer (“vermicompost”).
The fungal activity on the hair gives a degraded organic material and an extract with a pool of proteases with, amongst others, keratinolytic activity,. The enzyme activities expressed by the fungal extract are evaluated against soluble, insoluble, synthetic, and skin protein component substrates. Relationships between the different specific activities are defined to evaluate and predict the possible behaviour of the fungal extracts in the beamhouse processes.Note de contenu : - Solid state culture
- Objectives
- Experimental Development : Materials and Methods - Protocol of the analysis for activity assessment
- Results and Discussion : Assessment of enzymatic activities of crude extracts - Aminopeptidase Activity. Determination of kinetic constants - Assessment of the degree of hair digestion - Protein profile and Isoelectric Focusing (IEF) - Zymogram - Comments on mechanisms of fungal digestion of hair - Fungal extracts in the soaking, unhairing and bating processes. Possible applications
- Table 1 : Proteolytic activity of the fungal extracts of the different substrates
- Table 2 : Activity Relationships for Trichophyton ajelloi and Fusarium oxysporum fungal extractsEn ligne : https://drive.google.com/file/d/1Mrbx0x1BGf1ZNG_jsl1LMyTL1SX9KH-J/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39634
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 88, N° 3 (05-06/2004) . - p. 93-98[article]Exemplaires
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