[article]
| Titre : |
Multidimensional analysis of magnesium oxide hydrolysis of wet-blue shavings |
| Type de document : |
texte imprimé |
| Auteurs : |
Kalyani Martinelango, Auteur ; Dennis C. Shelly, Auteur |
| Année de publication : |
2004 |
| Article en page(s) : |
p. 258-268 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Magnesium oxide (MgO) hydrolysis is an established route to the conversion of chrome tanned leather waste to gelatin, protein hydrolysate and chrome cake. The objective of this work is to develop a deeper insight into MgO hydrolysis and to provide a foundation for developing new strategies for exploiting the products. A molecular level characterization of these protein materials was sought through a multidimensional analytical approach. Separation techniques such as Isoelectric Focussing (IEF), Hydrophobic Interaction Chromatography (HIC) and Reversed Phase High Performance Liquid Chromatography (RPHPLC) were combined with Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS). MALDI-TOF, itself, is a very useful analytical tool for characterizing large biomolecules such as oligopeptides and proteins. These analytical combinations proved useful in studying the product compositions of MgO hydrolysis. Generally, the hydrolysis produced many small, unremarkable peptides and relatively few larger fragments. Overall, there was good agreement among the molecular weight-based analytical techniques, across the separation and detection data domains. The very best analytical strategy would seem to be on-line coupling of RPHPLC and MS, providing high resolution, real-time speciation of these hydrolysis mixtures. |
| En ligne : |
https://drive.google.com/file/d/1UOVi9HqjGSPVtGOUU5cWyOGDf_B8Ykuf/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4212 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCIX, N° 6 (06/2004) . - p. 258-268
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