Application of acid protease for eco-friendly pre-treatment of goat skin to improve antimicrobial finish using herbal natural extracts / Mona Vajpayee in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVIII, N° 6 (06/2023)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVIII, N° 6 (06/2023) . - p. 219-234 Titre : Application of acid protease for eco-friendly pre-treatment of goat skin to improve antimicrobial finish using herbal natural extracts Type de document : texte imprimé Auteurs : Mona Vajpayee, Auteur ; Mumal Singh, Auteur ; Hemen Dave, Auteur ; Lalita Ledwani, Auteur Année de publication : 2023 Article en page(s) : p. 219-234 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Antimicrobiens Caractérisation Cuirs et peaux -- Finition Cuirs et peaux de chèvres Extraits de plantes Peptidases Traitement enzymatique Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Due to its moisture retention capacity and huge surface area, leather is highly prone to microbial proliferation and biodeterioration; hence, leather products desired have an antimicrobial finish. In this study, acid protease enzyme pre-treatment of goat skin was utilized as an eco-friendly substitute for conventional wet-chemical processing. The treatment can impart the desired surface properties to improve the antimicrobial finish with natural extracts obtained from leaves of Azadirachta indica (Neem Tree), Ocimum sanctum (Holy Basil, Tulsi), and Camellia sinensis (Green Tea). The procedure was optimized for different process parameters, including enzyme concentration, pH, material to liquor ratio (MLR), treatment time, and temperature. The effect of the treatment on bulk and surface properties of the skin was characterized by weight loss analysis, X-Ray Diffraction (XRD), Thermal Gravimetric Analysis (TGA), and Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-Ray Photoelectron Spectroscopy (XPS), Water contact angle measurement, Scanning Electron Microscopy (SEM) respectively. The effect of the enzymatic treatment on organoleptic properties and the mechanical strength of the skin was also studied. The enzymatic treatment resulted in weight loss, and removal of non-collagen components, thus opening the fibrous collagen matrix of the skin. Hence, the skin treated with acid protease enzyme provides better affinity and accessibility for phytoactive compounds from the natural extracts and better attachment by electrostatic attachment due to an increase in surface functional groups after the enzymatic treatment compared to untreated skin. The effectiveness of the antimicrobial finish was measured as a zone of inhibition and with a modified Hohenstein test against test microorganisms E. coli and S. aureus.Azadirachta indica (Neem Tree) extract showed the highest inhibitory activity (97%) against E. coli, while the Ocimum sanctum (Holy Basil, Tulsi) extract exhibited the highest inhibitory activity (95%) against S. aureus. Note de contenu : - EXPERIMENTAL WORK : Material - Enzymatic treatment of goat skin samples with acid protease - Characterization - Preparation of antimicrobial extract and antimicrobial finishing of goat skins - RESULTS AND DISCUSSION : Weight loss percentage - ATR-FTIR analysis - XPS analysis - TG and DTG analysis - XRD analysis - SEM analysis - Water contact angle measurement - Effect of the enzymatic treatment on mechanical strength and organoleptic properties - Effect of the enzymatic treatment on non-collagen components of the goat skin - Identification of active constituents of the natural extracts - Antimicrobial activity - Table 1 : XRD parameter of untreated and enzymatic treated goat skin - Table 2 : Results of mechanical strength analysis for untreated and enzyme-treated goat skin - Table 3 : Quantitative analysis of non-collagen skin components of the skin - Table 4 : Zone of inhibition (cm) obtained for untreated skin finished with the natural extracts (UT+EX) and the enzyme-treated skin finished with the natural extracts (ET+EX) - Table 5 : Percentage reduction of bacteria obtained by modified Hohenstein test for the enzyme-treated and untreated skin samples finished with the 5% w/v natural extracts; UT = Untreated skin, ET = skin with the enzymatic treatment DOI : https://doi.org/10.34314/jalca.v118i6.7588 En ligne : https://drive.google.com/file/d/1N60QBt1TbAhe30QdkSbZ-D1G85Sbw_1P/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39479 [article]

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