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A novel and efficient collagen extraction method assisted by microwave irradiation / Jinwei Zhang in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVIII, N° 5 (05/2023)
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Titre : A novel and efficient collagen extraction method assisted by microwave irradiation Type de document : texte imprimé Auteurs : Jinwei Zhang, Auteur ; Yulin Cheng, Auteur ; Jiacheng Wu, Auteur ; Wuyong Chen, Auteur Année de publication : 2023 Article en page(s) : p. 199-207 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Collagène -- Analyse
Cuirs et peaux de bovins
Extraction par micro-ondesIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : A new and efficient microwave heating method was developed to extract intact Type I collagen from bovine limed splits. Orthogonal experiment was conducted to optimize the parameters of extraction, and the extraction yield was measured by ultraviolet spectra (UV). The hierarchical structures of the obtained collagen were determined by amino acid analysis, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), UV, Fourier transform infrared spectra (FT-IR), circular dichroism spectra (CD), fluorescence spectra (FL) and ultra-sensitive differential scanning calorimetry (US-DSC). The results indicated the optimal conditions for this microwave assisted extraction were 37°C for 7 h by using solid to liquid ratio of 1:45. The resultant yield of collagen was 14.35% under the optimal conditions, which is 1.5 times higher than that obtained by the control water bath-heating method. Amino acid analysis, UV and SDS-PAGE revealed that the primary structure of collagen extracted with microwave assistance was type I collagen. Although the extraction yield was promoted drastically with microwave assisting, the secondary structure of the collagen involving triple helix still maintained according to FT-IR, CD, FL and VP-DSC results. In brief, microwave irradiation could be a new routine for collagen extraction with advantages of fast and effective. Moreover, this study might offer a potential choice for utilizing hide or skin limed split wastes. Note de contenu : - EXPERIMENTAL : Materials - Sample preparation - Testing methods - Sodium dodecyle sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) - Fourier transform spectroscopy measurement (FT-IR) - Circular dichroism spectroscopy measurement (CD) - Fluorecence spectroscopy measurement (FL) - Ultra-sensitive differential scanning calorimetry (US-DSC)
- RESULTS AND DISCUSSION : Optimization of microwave-assisting collagen extraction - Primary structure of collagen extracted by microwave assisted - Molecular weight of collagen - UV-Vis spectra of collagen - Secondary structure of collagen extracted by microwave assisted - CD spectra of collagen - The intrinsic fluorescence analysis of collagen - The synchronous fluorescence analysis of collagen - Denaturation temperature of collagen
- Table 1 : Orthogonal test results of collagen extraction by microwave irradiation
- Table 2 : Amino acid composition of different kinds of collagen
- Table 3 : Thermal denaturation temperatures of different kinds of collagenDOI : https://doi.org/10.34314/jalca.v118i5.7361 En ligne : https://drive.google.com/file/d/1NerQcNZ1n2lQQya7MdR_RQWXnQk2kzjB/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39305
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVIII, N° 5 (05/2023) . - p. 199-207[article]Réservation
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