Estimation of the sulfated glycosaminoglycan content of bovine skin with alcian blue / S. M. Mozersky in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. XCVIII, N° 9 (09/2003)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCVIII, N° 9 (09/2003) . - p. 337-343 Titre : Estimation of the sulfated glycosaminoglycan content of bovine skin with alcian blue Type de document : texte imprimé Auteurs : S. M. Mozersky, Auteur ; Renée J. Wildermuth, Auteur ; William N. Marmer, Auteur Année de publication : 2003 Article en page(s) : p. 337-343 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Procedures selected from the work of Bjornsson and Karlsson1,2 for the quantitation of sulfated glycosaminoglycans with Alcian Blue were modified and adapted to the measurement of this type of polysaccharide in bovine skin. Modifications include: (1) pulverization of the skin under liquid nitrogen, (2) brief, gentle digestion of the skin with collagenase, (3) the use of a variety of Alcian Blue widely used in histologic work, (4) the recipe for the preparation of the Alcian Blue reagent solution, (5) determination of suitable sample size, volumes of reagent solutions, incubation times, duration of centrifugation, etc. The procedure developed yields linear results for 50-300 mg samples of skin; the relative standard deviation for the procedure is +-15%. En ligne : https://drive.google.com/file/d/169-ps8Qurgctmtc1WnaY7zuxIjHz9jh_/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4149 [article]

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Immunochemical estimation of the decorin core-protein content of preparations of bovine decorin / S. M. Mozersky in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. XCIX, N° 7 (07/2004)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCIX, N° 7 (07/2004) . - p. 280-284 Titre : Immunochemical estimation of the decorin core-protein content of preparations of bovine decorin Type de document : texte imprimé Auteurs : S. M. Mozersky, Auteur ; William N. Marmer, Auteur ; Renée J. Wildermuth, Auteur Année de publication : 2004 Article en page(s) : p. 280-284 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : An indirect sandwich ELISA procedure has been developed for estimation of the decorin core-protein content of preparations of bovine decorin. A mouse anti-bovine decorin antibody is used as the capture antibody, and a rabbit anti-bovine decorin antibody as the detecting antibody. Goat anti-rabbit immunoglobulin G (IgG) labeled with horseradish peroxidase is used to react with the detecting antibody. The peroxidase substrate orthophenylenediamine (OPD), when exposed to the (bound) enzyme in the presence of peroxide, yields a soluble product with an absorbance maximum (in acidic medium) at 492 nm. The absorbance of the solution at this wavelength thus reflects the amount of decorin core-protein in the decorin preparation that is recognized by both the capture antibody and the detecting antibody. En ligne : https://drive.google.com/file/d/1BCbfy6ors4xeZRNi2Y_48JCXhAGVhMqz/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4214 [article]

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