Reproducible method for assessing the effects of blue light using in vitro human skin tissues / Rishabh Kala in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 1 (02/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 1 (02/2023) . - p. 95-107 Titre : Reproducible method for assessing the effects of blue light using in vitro human skin tissues Type de document : document électronique Auteurs : Rishabh Kala, Auteur ; Nicole Heiberger, Auteur ; Heather Mallin, Auteur ; Stephanie Wheeler, Auteur ; Anna Langerveld, Auteur Année de publication : 2023 Article en page(s) : p. 95-107 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Elisa, Test Le test ELISA est une méthode de laboratoire qualifiée d'immuno-enzymatique. Il s'agit d'un test immunologique, car il a pour principal objectif de mettre en évidence la présence d'anticorps ou d'antigènes spécifiques à une pathologie, dans un échantillon de sang. Cet examen utilise une protéine appelée enzyme, qui va se fixer à certains constituants spécifiques de la maladie, et par repérage et quantification de cette enzyme, la maladie pourra être affirmée, ainsi que son intensité évaluée. Expression génique Lumière bleue Peau -- Inflammation Peau -- Maladies Stress oxydatif Vieillissement cutané Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Introduction : High-intensity visible light (HEV), also referred to as blue light, has a wavelength of 400–500 nm and accounts for approximately one-third of the visible light. Blue light is also emitted from electronic devices and artificial indoor lighting. Studies have shown that exposure of human skin cells to light emitted from electronic devices, even as short as 1 h, can cause an increase in reactive oxygen species (ROS), apoptosis and necrosis. Despite comprising a significant portion of the light spectrum, the effects of HEV light have not been studied as extensively. This is in part due to a lack of suitable in vitro testing methods. This work was conducted in order to develop a reproducible testing method for assessing the effects of blue light on the skin. - Methods : Testing was performed using a full thickness, 3D in vitro skin tissue model. Different exposure protocols were tested to (1) determine the biological effects of blue light on the skin and (2) to identify an appropriate exposure for routine testing of cosmetic materials that may protect the skin from blue light damage. Gene expression and protein biomarkers were measured using qPCR, ELISA and immunohistochemical (IHC) methods. - Results : Our work demonstrates that daily exposure to blue light produced dose-and-time-dependent changes in biomarkers associated with skin damage. Exposure to blue light for 6 h for 5 consecutive days (total intensity of 30 J/cm2) increased the expression of genes that regulate inflammation and oxidative stress pathways and decreased the expression of genes that maintain skin barrier and tissue integrity. Exposure to blue light significantly increased protein biomarkers associated with ageing, inflammation and tissue damage. IHC staining confirmed changes in collagen, filaggrin and NQO1 protein expression. Treatment with ascorbic acid inhibited the effects of blue light, demonstrating a role in protection from blue light. - Conclusion : Our results showed that consistent blue light exposure produced skin damage via alterations in biological pathways that are associated with skin ageing. This work provides a new, reproducible in vitro testing method for assessing the effects of blue light on human skin using gene expression, protein ELISA and IHC staining. Note de contenu : - MATERIALS AND METHODS : In vitro skin tissues - Treatment of skin tissues - RNA isolation and quantitative reverse Transcription-polymerase chain reaction (qRT-PCR) analysis - Enzyme-linked immunosorbent (ELISA) protein assays - Immunohistochemistry (IHC) staining - RESULTS AND DISCUSSION : Blue light produced dose-dependent changes in gene expression - Blue light produced oxidative DNA damage - Blue light exposure caused an increase in skin ageing and MMP1 activity - Blue light exposure caused an increase in pro-inflammatory cytokine expression - Vitamin C prevents blue light damage - Table 1 : Statistically significant (unpaired t test, p < 0.05, N = 4) changes in gene expression are shown in Table 1 for each group vs the unexposed group DOI : https://doi.org/10.1111/ics.12821 En ligne : https://drive.google.com/file/d/15yOdVPKCyBpYYMPGCXgl8r5MER1WHETA/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38823 [article]

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