Arnica montana L. extract containing 6-O-methacryloylhelenalin and 6-O-isobutyrylhelenalin accelerates growth and differentiation of human subcutaneous preadipocytes and leads volumizing of skin / Kotaro Sakamoto in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 1 (02/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 1 (02/2023) . - p. 1-13 Titre : Arnica montana L. extract containing 6-O-methacryloylhelenalin and 6-O-isobutyrylhelenalin accelerates growth and differentiation of human subcutaneous preadipocytes and leads volumizing of skin Type de document : document électronique Auteurs : Kotaro Sakamoto, Auteur ; Chiharu Watanabe, Auteur ; Teruaki Masutani, Auteur ; Asuka Hirasawa, Auteur ; Kanae Wakamatsu, Auteur ; Arunasiri Iddamalgoda, Auteur ; Yuya Kakumu, Auteur ; Kosei Yamauchi, Auteur ; Tohru Mitsunaga, Auteur Année de publication : 2023 Article en page(s) : p. 1-13 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Adipocytes Antiâge:Antirides Arnica et constituants Arnica est un genre d'environ 30 espèces de plantes pérennes, herbacées, appartenant à la famille des Asteraceae. Ce genre montagnard se développe essentiellement dans les régions tempérées néarctiques (Amérique du Nord). Seules deux espèces sont natives des régions paléarctiques (Eurasie) : Arnica angustifolia et Arnica montana. En médecine traditionnelle, l'usage de l'arnica des montagnes est décrit dans des pharmacopées européennes pour son usage sur le traitement de petits traumatismes comme les hématomes, troubles menstruels, mais cet usage n'est pas soutenu par des études scientifiques. Cellules -- Cultures et milieux de culture Chimie analytique Dermo-cosmétologie Extraits de plantes:Extraits (pharmacie) Peau -- Soins et hygiène Repulpant (cosmétique) Tests de sécurité Index. décimale : 668.5 Parfums et cosmétiques Résumé : Objective : An important factor in the aging of the face is a reduction in the volume of adipose tissue. This reduction in adipose tissue contributes to decreased skin elasticity, which is also part of the aging process. Overall, these lead to wrinkle formation. Fat injection is a common means of addressing this issue and is used to reduce the effects of aging on the face and to increase the fullness of the lips and breasts. However, fat injection is an invasive surgical procedure. This study aimed to discover novel cosmetic ingredients that increase the volume of subcutaneous (pre)adipocytes to create the appearance of more youthful skin. - Methods : We focused on the number of subcutaneous preadipocytes and the accumulation of lipid droplets. To discover natural ingredients that increase both of these, extracts of 380 natural products were prepared and screened for their effects on both growth and differentiation (i.e., lipid droplet accumulation) of human subcutaneous preadipocytes. One extract was found to have the desired effects, and this was further studied to determine the active compounds. We then evaluated its efficacy in a human clinical study. - Results : We found that Arnica montana L. flower extract (AFE) accelerates both the growth and the differentiation of human subcutaneous preadipocytes. AFE was found to significantly increase the volume of adipocyte spheroids. The active compounds 6-O-methacryloylhelenalin and 6-O-isobutyrylhelenalin were found to be responsible for the effects of AFE on preadipocytes. In a human clinical study, gels containing 1% AFE successfully enhanced the volume of the lips and face with reduction of wrinkles with no adverse reactions. - Conclusion : This is the first report to demonstrate that AFE and the included compounds, 6-O-methacryloylhelenalin and 6-O-isobutyrylhelenalin, act on preadipocytes. AFE would be ideal for use in products that plump the face to reduce wrinkles and create a more youthful appearance. Note de contenu : - MATERIALS AND METHODS : Plant extracts preparation - In vitro cell-based assay - Evaluation of adiponectin secretion - Preparation of cell spheroids - Evaluation of lipid droplet amount in spheroids - Fractionation and identification of active compounds of AFE - Ethics statement - Chemical trials - Statistics analysis - RESULTS : Screening of 380 natural extracts for their effects on the growth and differentiation of preadipocytes - AFE activation of subcutaneous preadipocytes - AFE volumizing effects on adipocytes spheroids (3D-cultured cells) - Active compounds responsible for the effects of AFE on preadipocytes - Volumizing and wrinkle-reducing effects of AFE on human skin DOI : https://doi.org/10.1111/ics.12815 En ligne : https://drive.google.com/file/d/1a9DVvVwVnqDJA4DkGFZOmqmp_Alfg-za/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38815 [article]

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