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Application of molecular techniques to identify bacteria isolated from the leather industry. Discussion / David Oppong in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CI, N° 4 (04/2006)
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Titre : Application of molecular techniques to identify bacteria isolated from the leather industry. Discussion Type de document : texte imprimé Auteurs : David Oppong, Auteur ; Stephen D. Bryant, Auteur ; Ravindran Rangarajan, Auteur ; Susan Steele, Auteur ; David Radwell, Auteur ; Lori Hyllengren, Auteur Année de publication : 2006 Article en page(s) : p. 140-145 Note générale : Bibliogr. Langues : Américain (ame) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : DNA-based methods are known to be accurate, precise and rapid in identifying bacteria but there is little information about the use of these techniques in the leather industry. A study was undertaken to investigate and characterize the bacterial contaminants of fresh hides using 16S ribosomal RNA sequencing techniques. The proteolytic activities of these isolates were also studied using different substrates. Organisms belonging to 16 different genera of bacteria were isolated, and many of the species found have not been previously reported for this environment. Some of the species of bacteria being reported for the first time in the tanning environment included Janibacter terrae, Acidovorax sp., Dietzia maris, Arthrobacter pr tophormiae, Comamonas sp., Brevibacterium lutescens, Jeotgalicoccus psychrophifus, Nocardiopsis sp., Delftia acidovorans, Pseudomonas cannabina, Pseudomonas fulgida and certain species of Aeromonas and Acinetobacter. Most species of bacteria found in the incoming process water were non-proteolytic. Analysis of fresh hides revealed a large number of both proteolytic and non-proteolytic bacteria. The proteolytic bacteria included several species of Aeromonas, Proteus vulgaris, and Shewanella putrefaciens. Pseudomonas sp. were common but not all of them showed proteolytic activity. Knowledge about the identities and proteolytic activities of these organisms that occur in this environment would provide a better way to design programs to control theirundesirable activities. En ligne : https://drive.google.com/file/d/1W6m6QBqQgEHmOjGdvy_Z9dJA7dyTqpd0/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4017
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