Identification and characterisation of Aspergillus sp. from leather / Muhammad Ali in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 105, N° 6 (11-12/2021)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 105, N° 6 (11-12/2021) . - p. 307-311 Titre : Identification and characterisation of Aspergillus sp. from leather Type de document : texte imprimé Auteurs : Muhammad Ali, Auteur ; Faiza Sharif, Auteur ; Ahmad Ali Shahid, Auteur ; Muhammad Adnan Zafar, Auteur ; Muhammad Asif, Auteur Année de publication : 2021 Article en page(s) : p. 307-311 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Aspergillus Caractérisation Champignons microscopiques -- Identification Champignons pathogènes Cuirs et peaux -- Détérioration Cuirs et peaux de bovins Cuirs et peaux de buffles Cuirs et peaux de chèvres Cuirs et peaux de moutons Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Species of the genus Aspergillus are commonly known to be responsible for the degradation of tanned leather. In this study five species of Aspergillus (A. fumigatus, A. niger, A. flavus and A. ochraceopetaliformis) were isolated from tanned leather pieces of cow, goat, sheep and buffalo respectively. Among the isolated species A. ochraceopetaliformis was the first time reported from Pakistan. These isolated fungal strains were initially identified by their morphological and microscopic studies (conidial formation, conidial shape, size, colour, vesicle shape etc.) and then by means of molecular tools including genomic DNA isolation, PCR amplifications, genome sequencing and sequence analysis. Sequences were submitted to NCBI GenBank and accession numbers were assigned by NCBI. Phylogenetic analysis was also performed in order to calculate the percentage similarity as well as variation among the isolated species. Note de contenu : - MATERIAL AND METHODS : Collection of leather samples - Isolation of mycoflora - Molecular identification DNA isolation and amplification - PCR amplification - ITS data analysis - RESULTS : Morphological and microscopic identification of pathogenic fungi - Colony - Microscopic features - Table 1 : PCR amplification - Table 2 : Morphology and microscopy of leather degrading species of the genus aspergillus En ligne : https://drive.google.com/file/d/1sDLJtpYdUcYk1U48YAMWdrXv4UTQZRmF/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36723 [article]

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