Carbon dioxide ameliorates reduced desquamation in dry scaly skin via protease activation / Satoko Fukagawa in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 42, N° 6 (12/2020)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 42, N° 6 (12/2020) . - p. 564-572 Titre : Carbon dioxide ameliorates reduced desquamation in dry scaly skin via protease activation Type de document : document électronique Auteurs : Satoko Fukagawa, Auteur ; Ayami Takahashi, Auteur ; Keimon Sayama, Auteur ; Shinobu Mori, Auteur ; Takatoshi Murase, Auteur Année de publication : 2020 Article en page(s) : p. 564-572 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Dermo-cosmétologie Desquamation Dioxyde de carbone Peau -- analyse Peau -- Soins et hygiène Peptidases Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Scaling, a phenomenon showing an abnormal detachment of the stratum corneum (SC) owing to desquamation dysfunction, is commonly observed in various skin diseases or xerotic skin due to ageing and low humidity. Therefore, it is considered that ameliorating the disturbed desquamatory process of the SC leads to improvement in scaling. Carbon dioxide (CO2) is known to be good for some skin diseases; however, the effect of CO2 on scaling and its mechanism are not sufficiently clear. We aimed to elucidate the effect of transepidermal application of CO2 on scaling and its mechanism of action. - Methods : Twenty healthy men with mild scaling on the cheeks were recruited for a double-blind, placebo-controlled, split-face study. They applied the formulation containing CO2 twice daily for 1 week. After the study, the SC was collected by tape stripping to analyse desquamatory protease activities and degradation of extracellular corneodesmosomes. Furthermore, the contribution of pH to proteolysis of the corneodesmosome by CO2 was evaluated using three-dimensional (3D) cultured epidermal models. - Results : The spectroscopic absorbance of tape strips, used as scaling indicators, was decreased, concomitantly with the amelioration of incomplete degradation of desmoglein-1, one of the main corneodesmosomal proteins, and activation of trypsin-like protease in the SC by transepidermal application of CO2. Experiments using 3D cultured epidermis showed that pH in the epidermal tissue was lowered by CO2, whereas a pH change was not observed with the application of the formulation containing hydrochloric acid, which was added to equalize the pH to that of the CO2 formulation. - Conclusion : The transcutaneous application of CO2 ameliorates reduced desquamatory process in xerotic skin, with concomitant mild acidification of the SC, thereby leading to improvement in scaling. Thus, CO2 may have an advantage of efficiently and safely counteracting scaling of various skin disorders. Note de contenu : - MATERIALS AND METHODS : Test samples - Subjects and study - Cell culture - Immunohistochemistry - Measurement of protease activities - In situ zymography - Western blot analysis - Measurement of pH - Statistical analysis - RESULTS : Effects of CO2 on the dry scaly skin condition of the cheeks - Effects of CO2 on the corneodesmosomal protein and desquamatory proteases of the cheeks - Effects of CO2 on the corneodesmosomal protein and desquamatory proteases of the 3D epidermal model - Effect of CO2 on pH in the 3D cultured epidermis - Table 1 : Antibodies used for immunohistochemistry and Western blot analysis - Table 2 : 2 Substrate and standard reagents used for the protease activity assay DOI : https://doi.org/10.1111/ics.12641 En ligne : https://drive.google.com/file/d/1LvCKfSMbieYG97kGwPntfr8pY7d5mYJa/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35422 [article]

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