| Titre : |
Depilation of skins by pure enzymes |
| Type de document : |
texte imprimé |
| Auteurs : |
S. Priya, Auteur ; A. Rajaram, Auteur ; R. Rajaram, Auteur ; T. Ramasami, Auteur |
| Année de publication : |
2008 |
| Article en page(s) : |
p. 214-221 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Cuirs et peaux
Epilage enzymatique
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Five pure enzymes (pepsin, trypsin, chymotrypsin, elastase and papain) were used indepilation of goatskins in order to find alternatives to current methods (involving lime and sulphide). The morphologies of depilated skins and hair roots were investigated by both optical microscopy and scanning electron microscopy.
There were differences in morphology depending on the possible region of attack of these enzymes at the hair root. Trypsin and elastase were very effective in unhairing with the latter having a generally extensive effect whilepapain was less so. Pepsin and chymotrypsin were ineffective. The capacity of the enzymes for depilation has also been analyzed in terms of the availability of suitable sites for each of them for cleavage of the various proteins at the hair root and their propensity to cleave has been analyzed through the proteomics tools of the Swiss-Prot program. The results have indicated that theoretical predictions can be correlated to the experimental observations on the depilation of the skin. |
| Note de contenu : |
- MATERIALS AND METHODS : 1) Treatment of skins with proteolytic enzymes - 2) Histology - 3) Scanning Electron Microscopy of depilated skins and hair roots - 4) Analysis of number of cleavage sites in the proteins based on specificity of enzyme action
- RESULTS : 1) Histological assessment of the depilation process - 2) SEM investigations of the structure of the enzyme treated skin - 3) SEM investigations of the structure of the hair root from enzyme treated skin - 4) Analysis of the number of cleavage sites in proteins and propensity for cleavage
- Fig. 1 : Histology of skins. Optical micrographs of sections of skin depilated b different enzymes and after scudding - fixed with formalin. Sections were stained with haematoxylin and eosin.
- Fig. 2 : Scanning Electron Micrographs (SEM) of skin surfaces depilated by enzymes
- Fig. 3 : SEMs of hair roots skin subjected to different enzyme treatments shown at both low and high magnification
- Table 1 : Cleavage sites available for proteolytic enzymes on proteins of the epidermis, hair and dermis
- Table 2 : Efficacy of enzymes trypsin and chymotrypsin in cleaving the proteins present in the epidermis, hair and dermis |
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in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 92, N° 5 (09-10/2008) . - p. 214-221
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