Titre : |
Hydrolysis of tannery fleshings using pancreatic enzymes : a biotechnological tool for solid waste management |
Type de document : |
texte imprimé |
Auteurs : |
S. Kumaraguru, Auteur ; C. Rose, Auteur ; T. P. Sastry, Auteur |
Année de publication : |
1998 |
Article en page(s) : |
p. 32-40 |
Note générale : |
Bibliogr. |
Langues : |
Américain (ame) |
Index. décimale : |
675 Technologie du cuir et de la fourrure |
Résumé : |
Fleshings, the major solid waste generated at the pretanning operations of leather processing, were hydrolysed using pancreatic enzymes with a view to evolve a simple method for solid waste management. The proteolytic activity of pancreatic homogenate with casein was found to be 80 units/ml. Fleshings, treated with pancreatic enzyme preparation showed a six fold increase in proteolysis against the control at the end of 7 days. The total protein content, collagen and the free fatty acids in the hydrolysate supernatant were 80.0, 10.64 and 72.86 mg/ml respectively. The optimum pH for the enzyme preparation was 8.5. The hydrolysis was observed by almost total liquifaction of the fleshing. |
Note de contenu : |
- MATERIALS AND METHODS : Raw material - Preparation of enzyme solution - Protein determination - Determination of hydroxyproline - Determination of activity by the casein - digestion method - Hydrolysis of tannery fleshings - Optimization of enzyme concentration - Time course hydrolysis of fleshings - Optimization of pH - Measurement of hydorlysis in the presence of Na2S2O5 - Determination of free fatty acids |
En ligne : |
https://drive.google.com/file/d/1Yq3FB7GoYIVXiu2WvlvktbhRJM42HhiH/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4522 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCIII, N° 2 (02-03/1998) . - p. 32-40