Factors affecting mass transfer of protease in pelt during enzymatic bating process / Ying Song in JOURNAL OF LEATHER SCIENCE AND ENGINEERING, Vol. 1 (Année 2019)  

[article]  inJOURNAL OF LEATHER SCIENCE AND ENGINEERING > Vol. 1 (Année 2019) . - 10 p. Titre : Factors affecting mass transfer of protease in pelt during enzymatic bating process Type de document : texte imprimé Auteurs : Ying Song, Auteur ; Siqi Wu, Auteur ; Qian Yang, Auteur ; Hao Liu, Auteur ; Yunhang Zeng, Auteur ; Bi Shi, Auteur Année de publication : 2019 Article en page(s) : 10 p. Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Confitage Le confitage est une action biochimique effectuée au moyen de produits enzymatiques, qui a pour but de dégrader les fibres élastiques, contribuant ainsi à augmenter la souplesse du cuir. En outre, les enzymes complètent la dégradation des résidus épidermiques, donnant ainsi une fleur plus propre et plus lisse. Cuirs et peaux Electrostatique Peptidases Poids moléculaires Tension superficielle Transfert de masse Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Bating pelt with protease is an important process, which removes unwanted non-collagenous proteins from the pelt and moderately disperse hide collagen network. However, the grain surface, may be excessively hydrolyzed during bating due to the longer retention time of protease in the grain than in the middle layer caused by the low mass transfer rate of protease in pelt. Here, the effects of protease dosage, common auxiliaries and molecular weight of protease on protease transfer during bating were investigated so that we can find the key points to avoid excessive collagen damage, particularly in the grain. Observably, increasing protease dosage led to faster protease transfer and softer leather, but along with more considerable grain damage. Using penetrating agent JFC (fatty alcohol-polyoxyethylene ether) and ammonium sulfate enhanced protease transfer and simultaneously alleviated collagen damage due to the decrease in interfacial tension and electrostatic attraction between protease and pelt, respectively. Additionally, proteases with lower molecular weight transferred faster in pelt, which suggests that a potential strategy to solve the conflict between the mass transfer and the reaction of protease in pelt might be to produce/employ smaller bating proteases. Note de contenu : - MATERIALS AND METHODS : Materials - Effect of protease dosage on bating performance - Effect of auxiliaries on bating performance of protease - Effect of molecular weight of protease on its transfer rate during bating - RESULTS AND DISCUSSION : The effect of protease dosage on bating performance - The effect of auxiliaries on bating performance - The effect of molecular weight of protease on its mass transfer rate during bating DOI : https://doi.org/10.1186/s42825-019-0007-7 En ligne : https://link.springer.com/content/pdf/10.1186/s42825-019-0007-7.pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36949 [article]

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Visualization of penetration and reaction of aldehyde tanning agent in leather using fluorescence technique / Ying Song in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXV, N° 7 (07/2020)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXV, N° 7 (07/2020) . - p. 248-254 Titre : Visualization of penetration and reaction of aldehyde tanning agent in leather using fluorescence technique Type de document : texte imprimé Auteurs : Ying Song, Auteur ; Siqi Wu, Auteur ; Ya-Nan Wang, Auteur ; Yudan Yi, Auteur ; Yunhang Zeng, Auteur ; Bi Shi, Auteur Année de publication : 2020 Article en page(s) : p. 248-254 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Agents de tannage Cuirs et peaux -- Analyse Glutaraldéhyde Pénétration (physique) Réactions chimiques Spectroscopie de fluorescence Tannage organique Index. décimale : 675.2 Préparation du cuir naturel. Tannage Résumé : Penetration and reaction of tanning agents in leather greatly influence tanning performance and leather quality. Aldehyde tanning agents are widely used in leather manufacture, but their penetration and reaction in leather cannot be detected easily. Considering the fact that aldehyde can form a fluorescent Schiff base with amino groups of collagen, we visualized the distribution and reactivity of typical aldehyde tanning agents in leather during tanning process using a fluorescence microscope. The results showed that glutaraldehyde in leather was easily observed under the fluorescein isothiocyanate (FITC) filter system. The fluorescence intensity of pelt/leather gradually increased with penetrating of glutaraldehyde, basifying and heating, which was consistent with the formation of Schiff base and the increase in Ts of pelt/leather. Oxidized sodium alginate and dialdehyde sodium carboxymethyl cellulose in leathers were also detected using a fluorescence microscope because the aldehyde group of the two tanning agents formed a fluorescent Schiff base with amino groups of collagen. The oxazolidine tanned leather and the tetrakis hydroxymethyl phosphonium tanned leather emitted little fluorescence because they crosslinked collagen with hydroxymethyl group. These results indicate the fluorescent technique is useful to visualize the penetration and reaction of aldehyde tanning agents that crosslink collagen with aldehyde groups. Note de contenu : - EXPERIMENTAL : Materials - Effect of glutaraldehyde crosslinking on fluorescence intensity of collagen - Determinations of penetration and reaction of glutaraldehyde in leather - Analyses of fluorescence intensity and Tg of the leathers tanned by different tanning - RESULTS AND DISCUSSION : Fluorescence emission spectra of GC - Penetration and reaction of glutaraldehyde in leather - Change in Tg of pelts/leathers during glutaraldehyde tanning process - Detection of other aldehyde tanning agents in leather DOI : https://doi.org/10.34314/jalca.v115i7.3835 En ligne : https://drive.google.com/file/d/1PdIgVX4Jfy5KYDKoEKiVPiF2pF9Ldfv1/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34326 [article]

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