New technologies for specific antiperspirant actions / Jessica Welzel in SOFW JOURNAL, Vol. 146, N° 3 (03/2020)  

[article]  inSOFW JOURNAL > Vol. 146, N° 3 (03/2020) . - p. 46-51 Titre : New technologies for specific antiperspirant actions Type de document : texte imprimé Auteurs : Jessica Welzel, Auteur ; Sabine Grüdl, Auteur ; Bernhard Banowski, Auteur ; Andrea Sättler, Auteur ; Thomas Förster, Auteur ; Thomas Welss, Auteur Année de publication : 2020 Article en page(s) : p. 46-51 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Aisselles -- Soins et hygiène Antiperspirants Cosmétiques Ingrédients cosmétiques Sels d'aluminium -- Produits de remplacement Index. décimale : 668.5 Parfums et cosmétiques Résumé : Cosmetics support a well-balanced quality of life of consumers by improving their outer appearance or repressing unpleasant attributes such as inordinate sweating or smelling. Especially sweating may be socially stigmatizing when becoming excessive. However, the biological mechanisms behind perspiration are not completely understood. The only effective cosmetic antiperspirant technology to reduce sweating is based on the use of aluminum salts, which lately became publicly misperceived. Henkel Beauty Care developed a sophisticated in vitro model of the human eccrine sweat gland to screen for and identify new antiperspirant technologies substituting these aluminum salts. This proprietary cell model combines all the relevant sweat gland cells in a three-dimensional (3D) environment. In verification tests of the cell model the in vitro simulation of both, stimulation of the sweating process and its inhibition, were shown. Further, this 3D model was used for screening of sweat-regulating actives. Thereby, we focused on the change of certain ion-levels within the cells upon treatment with potential inhibitors. Positively screened potential antiperspirant substances were subsequently tested in vivo revealing a striking in vitro / in vivo correlation. A new biologically potent antiperspirant technology is on its way to the consumer successfully replacing the common aluminum salts. Note de contenu : - 3D in vitro cell model of the human eccrine sweat gland - The in vitro screening - In vivo validation of in vitro observation - In vitro/in vivo correlation - Fig. 1 : Eccrine sweat gland cells in a suspension aggregate at the tip of the medium droplet in a Hanging Drop Plate forming the 3D eccrine sweat gland model - Fig. 2 : 2D cell culture (a) and 3D eccrine sweat gland model (b) in which the cells are loaded with an ion-sensitive fluorescence dye to measure the changes of intracellular ion contents. Bar represents 100 μm - Fig. 3 : Concentration dependent reduction of the intracellular sodium content after treatment of eccrine sweat gland cells with substance 1. ***: significantly different from 0% with p < 0.0001 (one-way ANOVA with Tukey’s post-hoc multiple comparison test). Data are mean ± SD of two independent experiments performed in sextuplet - Fig. 4 : Photograph of a volunteer’s back in a sweat reduction study during occlusion phase - Fig. 5 : Sweat reducing effect of test substance 1 determined on the back of volun-teers. Increasing concentrations of test substance 1 enhanced the sweat reducing potential up to the efficiency reached with conventionally used Aluminium chloro-hydrate (ACH). Data are mean of 15-20 volunteers - Fig. 6 : Observed correlation between the intracellular sodium content determined in substance 1-treated eccrine sweat gland cells in vitro and the actual sweat reducing efficiency of substance 1 achieved on the back of volunteers in vivo En ligne : https://drive.google.com/file/d/1uEVDnRC-MX-BGCnG11l8AZ0n5T6CuKaW/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33827 [article]

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A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology / Jessica Welzel in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 44, N° 2 (04/2022)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 2 (04/2022) . - p. 216-231 Titre : A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology Type de document : document électronique Auteurs : Jessica Welzel, Auteur ; Sabine Grüdl, Auteur ; Bernhard Banowski, Auteur ; Holger Stark, Auteur ; Andrea Sättler, Auteur ; Thomas Welss, Auteur Année de publication : 2022 Article en page(s) : p. 216-231 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Analyse génétique Cellules -- Cultures et milieux de culture Expression génique Glandes sudoripares Les glandes sudoripares (sudorales) sont des annexes cutanées (organes microscopiques spécialisés) qui sécrètent la sueur et, pour certaines d'entre elles, des hormones ou phéromones. Histologiquement, il s'agit de glandes exocrines (épithéliums cubiques bistratifiés) dont la fonction première est la transpiration. Les deux types de glandes sudoripares chez l'humain Chez l'humain, on distingue deux sortes de glandes sudoripares qui diffèrent par leur origine embryologique, leurs fonctions, leurs répartitions et par la composition de la sueur qu'elles excrètent. Glandes sudoripares « eccrines » Les glandes sudoripares eccrines sont de loin les plus nombreuses, de trois à six millions, avec une densité moyenne de 200 glandes/cm2. Leur topographie est quasi-ubiquitaire, avec une répartition sur presque l'ensemble de la surface cutanée, avec une plus forte densité au niveau de la paume des mains, de la plante des pieds (où elles atteignent une densité maximale de 600 glandes/cm2) et sur le front. Ces glandes sont absentes au niveau des petites lèvres et du clitoris chez les femmes, et du gland chez l'homme. Glandes sudoripares « apocrines » Chez l'humain, ces glandes se trouvent sous les aisselles (dans l'organe axillaire), sur la peau autour de l'anus et autour des mamelons. À la différence des glandes eccrines, la répartition des glandes apocrines est donc plus restreinte. Par ailleurs, les glandes apocrines présentent une plus grande taille, leur canal excréteur s'abouche dans un follicule pileux par lequel leur sécrétion est déversée ; celle-ci contient des lipides et des phéromones, des composés transformés par le microbiote bactérien cutané, produisant l'odeur de transpiration. Peau -- Histologie Virus Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Human eccrine sweat glands (eSG) represent vital components of the skin involved in regulating body temperature. Especially the eccrine duct, which opens directly into the skin surface and releases the aqueous sweat, constitutes the first contact point with topically applied substances. For scientific investigations and to understand the underlying sweating mechanism on a cellular level defined cellular material is beneficial. We, therefore, strived to generate a cell line derived from human eSG duct cells for identifying new mechanisms in sweating control, as such a standardized cell line is currently lacking. - Material and methods : Isolated primary human eSG duct cells were transduced with simian virus 40 large T antigen (SV40T) by lentiviral transduction. Successfully SV40T-transduced clones were selected by single-cell cloning with one clone, named 1D10, being particularly described in this work. - Results : In performed cellular investigations, SV40T-transduced duct-derived cells exhibited an extended lifespan with stable population doubling times suggesting its immortality. Besides, 1D10 clonal cell culture demonstrated similarities with parental, primary duct cells regarding gene expression of selected sweat gland-related markers. When combined with primary coil cells in a hanging drop co-culture, those transduced duct-derived cells showed some duct cell-like features. Further, a certain degree of cellular communication and a specific reaction towards substance application was observed. - Conclusion : Generated and herein described cell line derived from isolated human eSG duct cells is, based on the presented scientific findings, considered as immortal. Besides, this cell line shows some similarity with primary duct cells, although alterations from native glands were detected, among which is loss of expression of cystic fibrosis transmembrane conductance regulator. Provided some further investigations, presented SV40T-transduced duct-cell derived cell line seems a suited surrogate of primary eccrine duct cells. Note de contenu : - METHODS : Cell culture - Gene expression analysis - Histological examinations - RESULTS : Characteristics of SV40T-transduced duct-derived cells - Characteristics of clone 1D10 - DISCUSSION : Establishment of an immortalized eccrine sweat gland duct cell line - Characterization of SV40T-transduced eccrine sweat gland duct-derived cell clone 1D10 DOI : https://doi.org/10.1111/ics.12769 En ligne : https://drive.google.com/file/d/1LYIf5UT6xVWsFaaYEX2sxn2Anyn2OWhc/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37724 [article]

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