Effect of ionic liquids pretreatment on the extraction of collagen from calf skin / Sicong Liu in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIV, N° 10 (10/2019)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIV, N° 10 (10/2019) . - p. 391-399 Titre : Effect of ionic liquids pretreatment on the extraction of collagen from calf skin Type de document : texte imprimé Auteurs : Sicong Liu, Auteur ; Wentao Liu, Auteur ; Li Guoying, Auteur Année de publication : 2019 Article en page(s) : p. 391-399 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Chimie analytique Collagène Cuirs et peaux de veaux Extraction (chimie) Liquides ioniques Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The use of ionic liquids (ILs) for collagen extraction should be premised of not destroying the triple helix structure of collagen. Herein, the effects of pretreatments by two imidazolium based ILs with different anions, 1-ethyl-methylimidazolium dicyanamide ([EMIM][N(CN)2]) and 1-ethyl-methylimidazolium tetrafluoroborate ([EMIM][BF4]), on the extraction of collagen from calf skins were studied. The dependences of ILs pretreatments on ILs species and concentrations (30%, 50%, and 70% (w/w)) were examined, in terms of the fiber morphology of skins as well as the extraction rate, structural integrity, thermal stability, and aggregation behaviors of collagens. The results of histological analysis and scanning electron microscopy showed that the skin fibers were effectively loosened by the IL s pretreatments. The extraction rate of collagen was improved as the increase of ILs concentration and polarity with the highest value of 28.79%. Moreover, sodium dodecyl sulphatepolyacrylamide gel electrophoresis and Fourier transform infrared spectroscopy confirmed that the structural integrity of collagen was maintained after ILs pretreatments, although the thermal stability of collagen was determined to be slightly decreased by ultra-sensitive differential scanning calorimeter. Finally, pyrene fluorescence analysis and atomic force microscope indicated that the aggregation behavior of collagen was weakened when increasing the ILs concentration and polarity. The green ILs pretreatment of calf skins might be used as an effective approach for the extraction of bioactive collagen with improved yield and purity. Note de contenu : - EXPERIMENTAL : Materials - Ionic liquids pretreatment on NS - Histological analysis - SEM - Extraction of collagen - Extraction rate of collagen - SDS-PAGE - FTIR - Thermal analysis - Fluorescence measurements - AFM - RESULTS AND DISCUSSION : Effect of ILs on calf skin - Extraction rate anaysis - Structural integrity of collagen - Thermal stability of collagen - Aggregation behavior of collagen En ligne : https://drive.google.com/file/d/1IYTryFmWwzS6xJRTho0fxBzDFkzmgrxa/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33035 [article]

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Investigation of the solubility and dispersion degree of calf skin collagen in ionic liquids / Sicong Liu in JOURNAL OF LEATHER SCIENCE AND ENGINEERING, Vol. 1 (Année 2019)  

[article]  inJOURNAL OF LEATHER SCIENCE AND ENGINEERING > Vol. 1 (Année 2019) . - 12 p. Titre : Investigation of the solubility and dispersion degree of calf skin collagen in ionic liquids Type de document : texte imprimé Auteurs : Sicong Liu, Auteur ; Qian Li, Auteur ; Li Guoying, Auteur Année de publication : 2019 Article en page(s) : 12 p. Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bovins -- Races Chimie analytique Collagène Collagène -- Analyse Collagène -- Solubilité Dispersions et suspensions Liquides ioniques Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The dissolution of collagen in ionic liquids (ILs) was highly dependent on the polarity of ILs, which was influenced by their sorts and concentrations. Herein, the solubility and dispersion degree of collagen in two sorts of ILs, namely 1-ethyl-methylimidazolium tetrafluoroborate ([EMIM][BF4]) with low polarity and 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]) with high polarity in a concentration range from 10% to 70% at 10 °C were investigated. When 150 mg of collagen was added to 30 mg of ILs, the minimum soluble collagen concentration was 0.02 mg/mL in 70% [EMIM][BF4] with lowest polarity and the maximum was 3.57 mg/mL in 70% [EMIM][Ac] with highest polarity, which indicates that soluble collagen and insoluble collagen fibers were both present. For insoluble collagens, differential scanning calorimetry showed that the thermal-stability was weakened when increasing the ILs concentration and polarity, and the fiber arrangement was looser with a more uniform lyophilized structure, observed by atomic force microscopy and scanning electron microscopy. For soluble collagens, electrophoresis patterns and Fourier transform infrared spectroscopy showed that no polypeptide chain degradation occurred during dissolution, but the thermal denaturation temperature decreased by 0.26 °C~ 7.63 °C with the increase of ILs concentrations, measured by ultra-sensitive differential scanning calorimetry. Moreover, the aggregation of collagen molecules was reduced when ILs polarity was increased as determined by fluorescence measurements and dynamic light scattering, which resulted in an increased loose fiber arrangement observed by atomic force microscopy. If the structural integrity of collagen needs to be retained, then the ILs sorts and concentrations should be considered. Note de contenu : - EXPERIMENTAL : Materials - Processing collagen with ILs - Collagen solubility in ILs - DSC of insoluble collagen fibrils - SEM of insoluble collagen fibrils - AFM measurements of insoluble and soluble collagen - SDS–PAGE patterns of soluble collagen - FTIR of soluble collagen - Fluorescence measurements of soluble collagen - DLS measurements of soluble collagen - RESULTS AND DISCUSSION : Solubility of collagen in ILs - Thermal analysis of insoluble collagen fibrils - SEM images of insoluble collagen fibrils - AFM images of insoluble collagen fibrils - SDS-PAGE patterns of soluble collagen - FTIR spectra analysis of soluble collagen - Thermal analysis of soluble collagen - Fluorescence analysis of soluble collagen - DLS analysis of soluble collagen - AFM images of soluble collagen - Table 1 The concentration of soluble collagen in different ILs and concentrations - Table 2 Thermodynamic parameters of Col and collagen samples dissolved in different concentrations of [EMIM][Ac]a DOI : https://doi.org/10.1186/s42825-019-0013-9 En ligne : https://link.springer.com/content/pdf/10.1186/s42825-019-0013-9.pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36945 [article]

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