Leather species identification via mitochondrial DNA polymerase chain reaction / Xiang Zhang in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIV, N° 7 (07/2019)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIV, N° 7 (07/2019) . - p. 265-270 Titre : Leather species identification via mitochondrial DNA polymerase chain reaction Type de document : texte imprimé Auteurs : Xiang Zhang, Auteur ; Steve Lange, Auteur Année de publication : 2019 Article en page(s) : p. 265-270 Note générale : Bibliogr. Langues : Américain (ame) Catégories : ADN mitochondrial Cuirs et peaux -- Analyse Cuirs et peaux -- Identification Génétique animale Réaction en chaîne par polymérase Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Currently the identification of leather species is mainly via a skilled operator using optical microscopy and an extensive visual reference library. However, due to the limitations of optical microscopy and in cases where the leather surface is heavily corrected or finished, it is not always possible to accurately determine the leather species. Therefore, it is crucial to develop a reliable and easy to perform approach to overcome these limitations. In this paper, we used the unique DNA sequence present in every species to identify leather. The conversion process of making leather destroys much of the DNA present but the high copy number of mitochondrial DNA (mtDNA) in relation to nuclear DNA makes some mtDNA likely to survive and be detected via polymerase chain reaction (PCR). However, leather DNA is highly degraded and inhibitors present in the extracted DNA make successful PCR challenging. There are only a few related works reported in the literature attempting to use DNA for leather species identification and they provide limited technical details. In this study, species-specific primers for PCR were designed, conditions for DNA extraction from leather followed by PCR were optimized, and a detailed protocol was also provided. As a proof of concept, our approach demonstrated that the species of leather can be reliably detected via PCR targeting mtDNA. Note de contenu : - EXPERIMENTAL : Reference DNA - Leather DNA extraction - mtDNA sequence analysis and primer design - Leather species identification via mtDNA PCR - PCR product analysis via agarose gel electrophoresis - RESULTS AND DISCUSSION : Reference genomic DNA preparation - Leather DNA extraction - Species-specific primer design - Optimization of PCR condition En ligne : https://drive.google.com/file/d/1LSX8z8UCK5XOkjgexJmC7lyWH6zZR4KQ/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=32785 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
21086	-	Périodique	Bibliothèque principale	Documentaires	Disponible

---

1 (1 - 1 / 1)