| Titre : |
Immunochemical estimation of the decorin core-protein content of preparations of bovine decorin |
| Type de document : |
texte imprimé |
| Auteurs : |
S. M. Mozersky, Auteur ; William N. Marmer, Auteur ; Renée J. Wildermuth, Auteur |
| Année de publication : |
2004 |
| Article en page(s) : |
p. 280-284 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
An indirect sandwich ELISA procedure has been developed for estimation of the decorin core-protein content of preparations of bovine decorin. A mouse anti-bovine decorin antibody is used as the capture antibody, and a rabbit anti-bovine decorin antibody as the detecting antibody. Goat anti-rabbit immunoglobulin G (IgG) labeled with horseradish peroxidase is used to react with the detecting antibody. The peroxidase substrate orthophenylenediamine (OPD), when exposed to the (bound) enzyme in the presence of peroxide, yields a soluble product with an absorbance maximum (in acidic medium) at 492 nm. The absorbance of the solution at this wavelength thus reflects the amount of decorin core-protein in the decorin preparation that is recognized by both the capture antibody and the detecting antibody. |
| En ligne : |
https://drive.google.com/file/d/1BCbfy6ors4xeZRNi2Y_48JCXhAGVhMqz/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=4214 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. XCIX, N° 7 (07/2004) . - p. 280-284
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Immunochemical estimation of the decorin core-protein content of preparations of bovine decorin in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. XCIX, N° 7 (07/2004)
