[article]
| Titre : |
Melanosomal localization is required for GIF-2115/2250 to inhibit melanogenesis in B16F10 melanoma cells |
| Type de document : |
texte imprimé |
| Auteurs : |
Ayumi Sakurai, Auteur ; Kyoka Kawaguchi, Auteur ; Miyu Watanabe, Auteur ; Sayaka Okajima, Auteur ; Saho Furukawa, Auteur ; Kenichi Koga, Auteur ; Kentaro Oh-Hashi, Auteur ; Yoko Hirata, Auteur ; Kyoji Furuta, Auteur ; Hiroshi Takemori, Auteur |
| Année de publication : |
2024 |
| Article en page(s) : |
p. 668-679 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Cellules -- Cultures et milieux de culture
Chimie analytique
Dermo-cosmétologie
Mélanosome
OrganitesLes organites (de temps en temps appelés organelles par anglicisme) sont des structures spécialisées contenues dans le cytoplasme et séparées du reste de la cellule par une membrane phospholipidique. Il existe de nombreux types d'organite, en particulier dans les cellules eucaryotes. On a longtemps cru les organites absents des cellules procaryotes, mais quelques exceptions ont été mises en évidence. (Wikipedia)
Peau -- Soins et hygiène
Pigmentation, Troubles de la
Systèmes de livraison (pharmacie)
Tyrosinase
Vectorisation
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : Tyrosinase inhibitors suppress melanogenesis in melanocytes. During a screening for tyrosinase inhibitors, however, we noticed some discrepancies in inhibitory efficacies between melanocytes and in vitro assays. The compound (S)-N-{3-[4-(dimethylamino)phenyl]propyl}-N-methyl-indan-1-amine (GIF-2115) exerts antioxidative stress activity upon accumulation in late endosomes and lysosomes. GIF-2115 was also identified as a potent antimelanogenic reagent in B16F10 mouse melanoma cells. GIF-2115 inhibited the activity of mushroom tyrosinase and the lysates of B16F10 cells. However, structure–activity relationship studies indicated that GIF-2238, which lacks the benzene ring in the aminoindan structure of GIF-2115, inhibited tyrosinase activity in vitro but did not inhibit melanogenesis in B16F10 cells. The aim of the present study is to show the importance of the intracellular distribution of tyrosinase inhibitors in exerting their antimelanogenic activity in melanocytes.
- Methods : The intracellular distribution of compounds was monitored by linking with the fluorescent group of 7-nitro-2,1,3-benzoxadiazole (NBD). To mislocalize GIF-2115 to mitochondria, the mitochondria-preferring fluoroprobe ATTO565 was used.
- Results : We reconfirmed the localization of GIF-2250 (GIF-2115-NBD) not only to matured but also to early-stage melanosomes. Although GIF-2286 (GIF-2238-NBD) maintained tyrosinase inhibitory activity, it did not show specific intracellular localization. Moreover, when GIF-2115 was linked with ATTO565, the resultant compound GIF-2265 did not inhibit melanogenesis in B16F10 cells, despite its strong tyrosinase inhibitory activity.
- Conclusion : These results suggest that melanosomal localization is essential for the antimelanogenic activity of GIF-2115, and GIF-2115 derivatives may be new guides for drugs to endosomes and lysosomes as well as melanosomes. |
| Note de contenu : |
- MATERIALS AND METHODS :
- GIF compounds
- Cell culture and reagents
- Melanin assay
- Cell viability
- Tyrosinase activity
- mCherry-GRP143
- Co-localization assay
- Cell fractionation
- Statistical analysis
- RESULTS :
- GIF-2115 inhibits tyrosinase activity
- APDA structure is essential for tyrosinase inhibition but insufficient for the inhibition of melanin synthesis in B16F10 cells
- GIF-2115 is localized to melanosomes
- Mislocalization of GIF-2115 results in the loss of its inhibitory effect on melanin synthesis |
| DOI : |
https://doi.org/10.1111/ics.12949 |
| En ligne : |
https://drive.google.com/file/d/1PbXo9YgsEm7Nd7GqzwVZgjEo4HZAr6Yw/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=41562 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 46, N° 5 (10/2024) . - p. 668-679
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Melanosomal localization is required for GIF-2115/2250 to inhibit melanogenesis in B16F10 melanoma cells in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 46, N° 5 (10/2024)
