Extraction of anthocyanins from Aronia melanocarpa skin waste as a sustainable source of natural colorants / Muhammad H. Wathon in COLORATION TECHNOLOGY, Vol. 135, N° 1 (02/2019)  

[article]  inCOLORATION TECHNOLOGY > Vol. 135, N° 1 (02/2019) . - p. 5-16 Titre : Extraction of anthocyanins from Aronia melanocarpa skin waste as a sustainable source of natural colorants Type de document : texte imprimé Auteurs : Muhammad H. Wathon, Auteur ; Nicholas Beaumont, Auteur ; Meryem Benohoud, Auteur ; Richard S. Blackburn, Auteur ; Christopher M. Rayner, Auteur Année de publication : 2019 Article en page(s) : p. 5-16 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Adsorption Anthocyanes Colorants végétaux Extraction (chimie) Index. décimale : 667.2 Colorants et pigments Résumé : Aronia melanocarpa (Michx.) Elliott (black chokeberry) skin wastes from the production of Aronia fruit juice were extracted using a batch extraction method and a novel integrated extraction-adsorption process. Optimum conditions for batch extraction were as follows: 60 °C, 3 h, acid (0.1% v/v hydrochloric acid), biomass-to-solvent ratio of 1:16, and biomass-to-solid phase extraction resin ratio of 1:1. The integrated extraction-adsorption process gave improved anthocyanin yields of higher quality when the process was performed for 3 h without cooling of the circulating liquid, and with a flow rate of 1.3 ml s-1. Overall, the new method showed better anthocyanin yield and purity compared with the batch method, increasing the extraction yield by ca. 20% (5.25 6.34 mg g-1 dry weight of pomace) and increasing anthocyanin content by ca. 40% (19.9 28.4% w/w dry weight of extract). This method also simplified the process as three steps were eliminated saving time and energy. Furthermore, the integrated extraction-adsorption method is industrially scalable to produce large quantities of anthocyanins. In the batch method, anthocyanins present in A. melanocarpa skins were identified as cyanidin-3-O-galactoside (38.8%), cyanidin-3-O-arabinoside (6.4%), cyanidin-3-O-glucoside (3.6%), cyanidin-3-O-xyloside (0.5%), and the cyanidin aglycon (50.7%); in the continuous method, anthocyanin content was cyanidin-3-O-galactoside (45.7%), cyanidin-3-O-arabinoside (16%), cyanidin-3-O-glucoside (3.6%), cyanidin-3-O-xyloside (2.7%), and the cyanidin aglycon (32%). The integrated extraction-adsorption method was shown to be substantially less susceptible to acid-catalysed anthocyanin decomposition processes. All anthocyanins were derived from only one anthocyanidin parent structure (cyanidin), and only monosaccharide glycosides were identified, which is unusual when compared with other berries that typically have more anthocyanidins and/or greater glycosylation diversity. Note de contenu : - Chemicals and materials - General procedures and instrumentation - Sequential batch extraction - adsorption method - Integrated extraction-adsorption method - Fig. 1 : Effect of pH on anthocyanin structure and resultant colour - Fig. 2 : Experimental set-up for the integrated extraction-adsorption process. Arrows indicate direction of flow of extracted liquid - Fig. 3 : Extraction profiles during a batch extraction of Aronia melanocarpa waste skins at various temperatures. The absorbance was monitored using a UV-vis spectrometer at 520 nm - Fig. 4 : Extraction profiles during a batch extraction of Aronia melanocarpa waste skins at different pH (2.4 and 5.2) at 60°C. The absorbance was monitored a UV-vis spectrometer at 520 nm - Fig. 5 : Effect of pH on UV-vis absorbance of anthocyanins extracted from Aronia melanocarpa waste skins; measurement was carried out on post-SPE residues of th ethanol elute [1 mg extract in 1 ml acidified water (0.1% v/v hydrochloric acid)]. The colours of aqueous solutions tested are shown at the top - Fig. 6 : H-NMR spectra of post-SPE residues of ethyl acetate elute and ethanol elute. The 1H-NMR spectra were recorded at 500 MHz. Labels 1–5 refer to the five signals seen in the H NMR spectra of anthocyanins and correspond to their H-4 protons - Fig. 7 : The comparison between (left) a batch method and (right) an integrated extraction-adsorption method - Fig. 8 : Total anthocyanins calculated according to relative % shown in HPLC chromatograms of the batch method and the integrated method. Solvent A : H2O : TFA (99.5:0.5) ; solvent B : acetonitrile. Detected by DAD at 520 nm - Fig. 9 : Profile of anthocyanins shown in HPLC chromatograms of (a) the batch and (b) the integrated method. Solvent A: H2O/TFA (99.5:0.5) ; solvent B: acetonitrile. Detected by DAD at 520 nm : 1. Cy3gal ; 2. Cy3ara ; 3. Cy3glc ; 4. Cy3xyl ; 5. cyanidin (aglycon) ; 6. polymeric species - Fig. 10 : H-NMR spectra of post-SPE residues of ethanol wash for the batch method and the integrated method. The 1H-NMR spectra were recorded at 500 MHz. Labels 1–5 refer to the five signals seen in the H NMR spectra of anthocyanins and correspond to their H-4 protons - Fig. 11 : The effect of acid concentration on total anthocyanins calculated according to relative % shown in HPLC chromatograms of (a) the batch and (b) the integrated method. Solvent A : H2O/TFA (99.5:0.5) ; solvent B: acetonitrile. Detected by DAD at 520 nm DOI : 10.1111/cote.12385 En ligne : https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12385 Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=31660 [article]

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