[article]
| Titre : |
Examination of haloversatile bacteria on salted goatskin and inactivation of haloversatile bacteria via direct electric current |
| Type de document : |
texte imprimé |
| Auteurs : |
S. Kose, Auteur ; P. Yilmaz, Auteur ; Meral Birbir, Auteur ; Yasar Birbir, Auteur |
| Année de publication : |
2024 |
| Article en page(s) : |
p. 184-197 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Bactéries -- Comptage
Bactéries -- Identification
Bactéries haloversatiles
Caractérisation
Courants continus
Cuirs et peaux -- Analyse
Cuirs et peaux -- Détérioration
Cuirs et peaux de chèvres
Enzymes microbiennes
Peaux salées
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Haloversatile bacteria are among the commonly found microorganisms that have the potential to damage hides and skins in the leather industry. Therefore, the objective of this study was to investigate the presence of haloversatile bacteria on salted goatskins, to characterize these microorganisms through the use of molecular and conventional test methods, to detect their impact on the skins, and finally find an effective solution to inactivate these microorganisms. Haloversatile bacteria were common inhabitants at salted goatskin samples obtained from the Tuzla Organized Leather Industry Zone in Türkiye. Total numbers of haloversatile bacteria, proteolytic haloversatile bacteria, and lipolytic haloversatile bacteria on ten salted goatskin samples ranged from 7×104 to 2.7×105 CFU/g, 1×104 to 8×104 CFU/g, and 1×104 to 1.3×105 CFU/g, respectively. In the present study, 88% of the isolates were protease-positive, 69% were lipase-positive, 8% were xylanase-positive, 27% were caseinase-positive, 23% were amylase-positive, 8% were DNase-positive, 31% were cellulasepositive, 54% were urease-positive, 100% were catalase-positive, and 54% were oxidase-positive. The bacterial isolates showed positive reactions for the utilization of different amino acids such as glycine, L-cysteine, L-proline, and L-threonine, having the highest rates of 88%, 80%, 80%, and 80%, respectively. However, L-histidine had a lower positive reaction rate of 31%. The halophilic bacterial isolates exhibited positive reactions for the utilization and acid production from different types of sugar, with glucose having the highest positive reaction rate of 81%, followed by maltose at 73%, xylose at 58%, galactose at 46%, and lactose at 42%. Haloversatile enzymeproducing bacteria were identified using biochemical and molecular methods, resulting in the identification of 17 different species. Micrographs obtained from the scanning electron microscope revealed the damage inflicted on the fresh goatskin structure by haloversatile bacteria. A direct electric current of 2.2 A was applied to the mixed culture of haloversatile bacteria for 25 minutes to find an effective inactivation method. The total count of the mixed culture of haloversatile bacteria decreased from 7.3×106 CFU/mL to 4 CFU/mL within 16 minutes. All seventeen haloversatile bacteria in the mixed culture were killed within 19 minutes. |
| Note de contenu : |
- Collection of salted goatskin samples from tanneries and measurement of pH values
- Determination of the total numbers of haloversatile bacteria, proteolytic, and lipolytic haloversatile bacteria on the salted goatskin samples
- Isolation of haloversatile bacteria
- Haloversatile bacterial identification
- Determination of optimal growth conditions for haloversatile bacteria
- Investigation of enzymatic properties of haloversatile bacteria
- Utilization of different amino acids and carbon sources by haloversatile bacteria
- Examination of the damage caused by enzyme-producing haloversatile bacteria on the goatskin sample using scanning
electron microscope
- Destruction of haloversatile bacteria using electric current
- Examination of haloversatile bacterial cells before and after treatment with direct electric current using scanning electron microscopy
- Table 1 : The salted skin sample codes, ph of salted skin samples, total haloversatile, total proteolytic haloversatile, total lipolytic haloversatile bacterial counts on salted goatskin samples (CFU/g)
- Table 2 : The isolate codes, phylogenetically similar species, length (bp), similarity (%), accession number of haloversatile isolates obtained from salted goatskin samples
- Table 3 : The effects of pH, NaCl contents and temperature values on the growth of haloversatile bacterial isolates
- Table 4 : Enzymatic characteristics of haloversatile isolates obtained from salted goatskin samples
- Table 5 : Utilization of different sugars and amino acids by haloversatile isolates
- Table 6 : The pH values, temperature, voltage and the total counts (CFU/mL) of enzyme producing haloversatile bacteria in the direct electric current treatment |
| En ligne : |
https://drive.google.com/file/d/1WN-TWYW9l8-TRlCWsX0w8KSiISJyWWKx/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40826 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIX, N° 4 (04/2024) . - p. 184-197
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Examination of haloversatile bacteria on salted goatskin and inactivation of haloversatile bacteria via direct electric current in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIX, N° 4 (04/2024)
