| Titre : |
Hair saving unhairing process. Part 5 characterisation of enzymatic preparations applied in soaking and unhairing processes |
| Type de document : |
texte imprimé |
| Auteurs : |
Carlos S. Cantera, Auteur ; L. Goya, Auteur ; Betina Galarza, Auteur ; Maria Laura Garro, Auteur ; Laura M. I. Lopez, Auteur |
| Année de publication : |
2003 |
| Article en page(s) : |
p. 69-77 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Caractérisation
Epilage enzymatique
Peaux brutes -- Trempe
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Considering the importance that is attached to the characterisation of proteolytic enzymatic preparations employed in leather making, especially in the development of enzyme unhairing, our studies are aimed to develop a protocol of analysis that allows us to determine the behaviour of the enzymes on diverse protein skin substrates. In the protocol presented here it is important to highlight the incorporation of the so-called ‘epidermis substrate’, comprising the epidermis layer, hair and the root sheaths, as well as the procedure to assess enzymatic action on the proteoglycans.
On two of the substrates, “epidermis” and Hide Powder Azure, studies on activation/inhibition of the enzyme activity using components usually added in the soaking and unhairing processes was also carried out.
The protocol was applied to two commercial enzymatic preparations commonly used in leather processing, and to an extract of vegetable proteases that CITEC, jointly with LIPROVE, is applying in its investigation on enzyme unhairing.
The protocol developed is a useful tool to assist us in the design of ideal enzyme unhairing: that in which the enzymatic activity is orientated towards the components of the epidermis system, when their action on fibrous proteins – particularly collagen - is minimised or avoided and when the leathers developed have the desired properties. |
| Note de contenu : |
- INTRODUCTION : Chromogenic substrates - The epidermis layer, hair and root sheaths : the ‘epidermis substrate’ - Activity on proteoglycans (PGs) and hide powder substrate
- OBJECTIVES
- MATERIALS AND METHODS : 1) Assessment of protein content - 2) Assessment of activities on different substrates : Non – keratin based substrates - Activity upon proteoglycans (PGs). Hide powder substrate - Keratin-based substrates - 3) Inhibition and activation tests - 4) Protein Profile. Electrophoresis in SDS/polyacrylamide gel (PAGE) - 5) Isoelectric Focusing (IEF) - Zymogram
- EXPERIMENTAL RESULTS
- PROTEOLYTIC ACTIVITY AGAINST DIFFERENT SUBSTRATES AND ACTIVATION AND INHIBITION ASSAYS
- ANALYSIS OF THE RESULTS : Activation and Inhibition Tests
- Table 1 : Proteolytic activity of the enzymatic preparations against different substrates
- Table 2 : Ranges of pH corresponding to 90% of the maximum activity for each one of the enzymatic preparations (maximum activity pH value is shown in brackets)
- Table 3 : Changes in the activity of Alcamax and Pellvit against the chromogenic substrates hide powder azure and ‘epidermis’ adding components used in soaking and unhairing processes (*) |
| En ligne : |
https://drive.google.com/file/d/1lrLmDHNIHN7pji0LbISFfwO_LGJtTNU1/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39802 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 87, N° 2 (03-04/2003) . - p. 69-77
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Hair saving unhairing process. Part 5 characterisation of enzymatic preparations applied in soaking and unhairing processes in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 87, N° 2 (03-04/2003)
