[article]
| Titre : |
Protective effect of human amniotic membrane extract against hydrogen peroxide-induced oxidative damage in human dermal fibroblasts |
| Type de document : |
document électronique |
| Auteurs : |
Negin Talachi, Auteur ; Elaheh Afzal, Auteur ; Masoumeh Nouri, Auteur ; Saeid Abroun, Auteur ; Morteza Zarrabi, Auteur ; Hoda Jahandar, Auteur |
| Année de publication : |
2023 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Antiâge
Antioxydants
Cellules -- Cultures et milieux de culture
Dermo-cosmétologie
Fibroblastes
Membrane amniotique
Peau -- Soins et hygiène
Peroxyde d'hydrogèneLe peroxyde d'hydrogène (H2O2), communément appelé eau oxygénée ou encore perhydrol (appellation industrielle), est un composé chimique liquide et visqueux, aux puissantes propriétés oxydantes (il est aussi réducteur). C'est donc un agent blanchissant efficace qui sert de désinfectant et (à haute concentration) d'oxydant ou monergol dans les fusées spatiales.
Stress oxydatif
Vieillissement cutané -- Prévention
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : One of the main approaches to preventing skin ageing is to protect fibroblast cells from oxidative stress. The promoting effect of the human amniotic membrane extract (hAME) on re-epithelization, proliferation and migration of cells in wound healing has been already well studied. This experimental study aimed to investigate the antioxidant activity of hAME against hydrogen peroxide (H2O2)-induced dermal fibroblast damage.
- Methods : Here, to establish the ageing model, human foreskin fibroblasts (HFFs) were exposed to 200 μM H2O2 for 2 h. HFFs were treated with 0.1 mg/ml AME for 24 or 48 h before or/and after H2O2 exposure. A total of 48 h following the H2O2 treatment, we measured cell proliferation, viability, senescence-associated β-galactosidase (SA-β-Gal), antioxidants and preinflammatory cytokine (IL-6) levels, as well as the expression of senescence-associated genes (P53 and P21).
- Results : The obtained results indicated that under oxidative stress, AME significantly increased cellular viability and not only promoted the cell proliferation rate but also attenuated apoptotic induction condition (p < 0.001). AME also significantly reversed the SA-β-Gal levels induced by H2O2 (p < 0.001). Additionally, both pre- and post-treatment regimen by AME down-regulated the expression of senescence marker genes (p < 0.001). Moreover, AME declined different oxidative stress biomarkers such as superoxide dismutase and catalase and increased the glutathione amount.
- Conclusion : Altogether, our results indicated that AME had a remarkable antioxidant and antiageing activity as pre- and post-treatment regimen, pointing to this compound as a potential natural-based cosmeceutical agent to prevent and treat skin ageing conditions. |
| Note de contenu : |
- MATERIALS AND METHODS : Preparation of AME - Cell culture and treatment - Establishment of ageing condition - Evaluation of cellular viability and apoptosis - Detection of senescence-associated β-galactosidase (SA-β-gal) - Assessment of antioxidants levels - Superoxide dismutase - Catalase - Glutathione - Assessment of IL-6 level - RNA isolation and real-time PCR analysis - Statistical analysis
- RESULT : Establishment of H2O2-induced ageing in human fibroblasts - AME attenuates H2O2-induced ageing regimen, promotes proliferation and enhances cellular viability - AME protects human fibroblasts from oxidative stress-induced senescence - Antioxidative and anti-inflammatory effects of AME - H2O2-induced
ageing regimen is not associated with interleukin-6 expression |
| DOI : |
https://doi.org/10.1111/ics.12818 |
| En ligne : |
https://drive.google.com/file/d/16lcmBb9zjEzLQaKzp5_cWXTqu-hFJmm-/view?usp=share [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38821 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 1 (02/2023)
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Protective effect of human amniotic membrane extract against hydrogen peroxide-induced oxidative damage in human dermal fibroblasts in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 1 (02/2023)
