[article]
| Titre : |
Reactive dyes for living cells : Applications, artefacts, and some comparisons with textile dyeing |
| Type de document : |
texte imprimé |
| Auteurs : |
Richard W. Horobin, Auteur ; Juan C. Stockert, Auteur ; Hua Zhang, Auteur |
| Année de publication : |
2022 |
| Article en page(s) : |
p. 3-15 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Cellules
Colorants réactifs
Sondes fluorescentes
|
| Index. décimale : |
667.3 Teinture et impression des tissus |
| Résumé : |
An inclusive chemical definition of “reactive” dyeing of textiles is introduced, encompassing the CI Azoic, CI Mordant, CI Reactive, CI Sulphur and CI Vat dye application classes. Such reactive dyeing increases fibre retention of dye and makes application practically possible. The analogous application of dyes and fluorescent probes as microscopic stains in biology and medicine is outlined, focussing on using reactive fluorescent probes with living cells. Parallels with textile dyeing are noted, eg, enhanced probe retention and facilitation of probe application. However, the primary purpose of using reactive probes with live cells is detection of properties of biological systems : to identify biological structures and chemical/biochemical contents ; assess biological functions and physicochemical properties; and determine changes in locations of cells and cell components. Problems occurring with such probes are outlined, particularly the problematic character of many standard protocols, and localisation artefacts arising with reactive probes whose reactant and product species are physiochemically significantly different. This latter problem is explored via a case study of possible reactant/product artefacts with probes for reactive oxygen species. Comparison of experimental observations of probe localisations with the localisations predicted using quantitative structure activity (QSAR) modelling indicates that such artefacts can occur with a significant proportion of chemically diverse, widely used, commercially available probes, as well as with experimental compounds reported in the literature. A graphical flowchart is provided to assess possible occurrence of reactant/product artefacts arising with reactive fluorescent probes localising in various organelles of living cells. |
| Note de contenu : |
- Contrasting perspectives on “reactive” dyeing of textiles
- Dyes and fluorescent probes used as microscopic stains in biology and medicine
- Why are some fluorescent probes reactive ?
- Problems arising with reactive fluorescent probes applied to live cells
- Standard protocols are more problematic than commonly assumed
- Consequences of physicochemical differences between a reactant and its product
- Case study of possible reactant/product artefacts arising with probes for reactive oxygen species
- Predicting the occurrence of reactant/product localisation artefacts
- Assessing occurrence and frequency of reactant/product localisation artefacts
- Table 1 : Some illustrative examples of using fluorescent probes to obtain information concerning living biological systems
- Table 2 : Pay-offs of reactivity for different dye classes in textile dyeing, and for different probe types in investigations of living cells and tissues
- Table 3 : How differences in physicochemical properties influence cellular localisation of MTT Tetrazolium and MTT Formazan, the reactant and product species of a reactive probe |
| DOI : |
https://doi.org/10.1111/cote.12577 |
| En ligne : |
https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12577 |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37494 |
in COLORATION TECHNOLOGY > Vol. 138, N° 1 (02/2022) . - p. 3-15
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Reactive dyes for living cells : Applications, artefacts, and some comparisons with textile dyeing in COLORATION TECHNOLOGY, Vol. 138, N° 1 (02/2022)
