| Titre : |
Screening of additives to reduce grain damage risk on unhairing by proteinase K |
| Type de document : |
texte imprimé |
| Auteurs : |
Mei Chen, Auteur ; Mingfang Jiang, Auteur ; He Li, Auteur ; Cheng Haiming, Auteur |
| Année de publication : |
2020 |
| Article en page(s) : |
9 p. |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Additifs
Criblage
Cuirs et peaux de bovins
Epilage enzymatique
Hydrolyse enzymatique
Inhibiteurs (chimie)
Ions métalliques
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Enzymatic unhairing is a cleaner strategy for leather-making. It is a potential alternative to the traditional hair-burning process. However, several shortcomings, such as uncontrolled enzymatic reaction, and risk of grain looseness and damage have restricted the broad application of enzymatic unhairing. In this work, metal ions and organic additives were screened for lessening the hydrolytic activity of proteinase K to collagen fiber. Then, the selected additives were applied to the enzymatic unhairing process for bovine hide. The results showed that a suitable concentration of metal ions (Cu (II), Fe (III) and Al (III)) and organic additives (salicylate, laurate, adipate, gallate and epicatechin (ECG)) could diminish approximately 35% of the hydrolytic activity of proteinase K to collagen fibers. Then, the additives were applied for the bovine hide enzymatic unhairing process. Hydroxyproline determination in the unhairing float shows that applying additives could reduce collagen hydrolysis. The morphology results showed that the grain damage could be significantly reduced with the addition of the screened additives in the proteinase K enzymatic unhairing system, whereas the addition of ECG and gallate significantly slowed down the unhairing speed. This outcome provides new potential to reduce the risk of grain damage in enzymatic unhairing process. |
| Note de contenu : |
- MATERIALS AND METHODS : Materials - Enzymatic activity assays - Effect of additives on hydrolytic activities of proteinase K - Determination of total protein (TP) content - Determination of hydroxyproline (Hyp) content - Scanning electron microscopy (SEM)
- RESULTS AND DISCUSSION : Screening of additives - Enzymatic unharing by proteinase K with additives
- Table 1 : The effect of the additives on the hydrolytic activity of proteinase K
- Table 2 : The results of enzymatic unhairing by proteinase K (150 U/g hide) with additives at 35 °C, pH 8.0 |
| DOI : |
https://doi.org/10.1186/s42825-020-00032-1 |
| En ligne : |
https://link.springer.com/content/pdf/10.1186/s42825-020-00032-1.pdf |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37451 |
in JOURNAL OF LEATHER SCIENCE AND ENGINEERING > Vol. 2 (Année 2020) . - 9 p.
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Screening of additives to reduce grain damage risk on unhairing by proteinase K in JOURNAL OF LEATHER SCIENCE AND ENGINEERING, Vol. 2 (Année 2020)
