Titre : |
Developing new marine antifouling surfaces : Learning from single strain laboratory tests |
Type de document : |
texte imprimé |
Auteurs : |
Sara I. Faria, Auteur ; Luciana C. Gomes, Auteur ; Rita Teixeira-Santos, Auteur ; Joao Morais, Auteur ; Vitor Vasconcelos, Auteur |
Année de publication : |
2021 |
Article en page(s) : |
p. 21-28 |
Note générale : |
Bibliogr. |
Langues : |
Anglais (eng) |
Catégories : |
Bactéries Biofilms Marines (peinture) Revêtements antisalissures:Peinture antisalissures Revêtements organiques Salissures biologiques Statistique Traîtements de surface
|
Index. décimale : |
667.9 Revêtements et enduits |
Résumé : |
The development of antifouling (AF) technology for marine environments is an area of intense research given the severe economic and ecological effects of marine biofouling. Preliminary data from in vitro assays is frequently used to screen the performance of AF coatings. It is intuitive that microbial composition plays a major role in surface colonisation. The rationale behind this study is to investigate whether using a mixed population for the in vitro tests yields substantially different results than using single strains during initial screening. A polymeric coating was tested against single- and dual-species cultures of two common microfouler organisms for 49 days. A bacterium (Pseudoaltermonas tunicata) and a cyanobacterium (Cyanobium sp. LEGE 10375) were used in this study. Linear regression analysis revealed that Cyanobium sp. biofilms were significantly associated with a higher number of cells, wet weight, thickness and biovolume compared to dual-species biofilms. P tunicata alone had a biofilm growth kinetics similar to dual-species biofilms, although the Ptunicata-Cyanobium sp. mixture developed less dense and thinner biofilms compared to both single-species biofilms. Cyanobium sp. LEGE 10375 biofilms provided the worst-case scenario, ie, the conditions that caused higher biofilm amounts on the surface material under test. Therefore, it is likely that assessing the AF performance of new coatings using the most stringent conditions may yield more robust results than using a mixed population, as competition between microfouler organisms may reduce the biofilm formation capacity of the consortium. |
Note de contenu : |
- Surface preparation
- Marine organisms and culture conditions
- Single- and dual-species biofilm formation
- Biofilm analysis
- Cell density and wet weight
- Thickness
- Confocal Laser Scanning Microscopy (CLSM)
- Statistical analysis |
En ligne : |
https://drive.google.com/file/d/1lZQHQHA90dLblel9g9zUfYnyPA2xX3kF/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36760 |
in POLYMERS PAINT COLOUR JOURNAL - PPCJ > Vol. 211, N° 4668 (04/2021) . - p. 21-28