[article]
| Titre : |
Examination of moderately halophilic bacteria |
| Type de document : |
texte imprimé |
| Auteurs : |
Meral Birbir, Auteur ; Kubra Kizilkaya, Auteur ; Pinar Caglayan, Auteur |
| Année de publication : |
2021 |
| Article en page(s) : |
p. 231-241 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Bactéries -- Comptage
Bactéries halophiles
Cendres
Cuirs et peaux -- Conservation
Cuirs et peaux -- Microbiologie
Humidité
Peaux brutes -- Salage
pH
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
The salt curing process may cause contamination of skin with micro-organisms found in the salt. Moderately halophilic bacteria are among the dominant halophilic micro-organisms which adversely affect skin quality. Therefore, the study was focused to examine prevalence and total numbers of aerobic bacteria, moderately halophilic bacteria, proteolytic and lipolytic moderately halophilic bacteria in curing salt samples and examine coiony characteristics and biochemical reactions of moderately halophilic bacteria. Twenty-five salt sampies that are used in the preservation of hides and skins were collected from different leather factories in Corlu (20 salt sampies) and Tuzla (5 salt samples) Leather Organized Industrial Zones in Turkey. The pH, ash and moisture values of the salt sampies were respectively determined as between 6.60-7.95, 90%-96%, 2.1%-2.9%. The total numbers of aerobic bacteria, moderately halophilic bacteria, proteolytic and lipolytic moderately halophilic bacteria in the sampies were detected as 2-70 CFU/g, 1-13 CFU/g, 1-13 CFU/g and 1-4 CFU/g, respectively. Ninety-five moderately halophilic bacterial isolates were recovered from the salt sampies. Three, thirteen, seventeen, fifty-six, two, four, five percent of the bacterial colonies were dark yellow, pink, white, cream, beige, dark cream and yellow, respectively. Seventy-nine, sixteen, four and one percent of the bacterial colonies were found as circuiar, punctiform, irregular and rhizoid, respectively. Sixty-six, thirty-four, forty-five and fifty-five percent of the isolates were Gram-positive, Gram-negative, cocci-shaped, and rod-shaped cells. Fifty-four and thirty-eight percent of the isolates were motile and endospore-forming bacteria, respectively. Ail isolates grew on complex agar media containing 3-15% NaCI (w/v) concentration but did not grow on complex agar medium without NaCI and complex agar medium with 30% NaCI. White optimal growth of eighty-two percent of the isolates were observed at Complex agar medium with 10% NaCI, eighteen percent of isolates exhibited optimal growth at Complex agar medium with 10-15% NaCI. All isolates were catalase-positive. Sixty-one, seventy-four, twenty-five, sixty percent of the isolates produced oxidase, protease, lipase, and amylase enzymes, respectively. Thirty-three of percent of the isolates produced ammonia from peptone. ln conclusion, ail salt sampies contained moderately halophilic bacteria, 92% and 48% of salt samples comprised respectively proteolytic and lipolytic moderately halophilic bacteria. Therefore, destructive moderately halophilic bacteria in each salt should be removed using antimicrobial applications before curing process to prevent damage to the salted hides and skins during storage. |
| Note de contenu : |
- Salt samples
- Determination of pH values, moisture and ash contents of salt samples
- Determination of numbers of aerobic bacteria, moderately halohilic bacteria, proteolytic moderately halophilic bacteria and lipolytic moderately halophilic bacteria in salt samples
- Biochemical ativities of moderately halophilic bacterial isolates
- Table 1 : The pH values, ash contents, moisture content, total counts of aerobic bacteria and moderately halophilic bacteria of the salt samples
- Table 2 : Bacterial colony characteristics of pure moderately halophilic bacterial isolates
- Table 3 : Biochemical test results of moderately halophilic bacterial isolates |
| En ligne : |
https://drive.google.com/file/d/1fiuvgEaAYneIoA1jwQhL4a7iKewvC3nP/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36349 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 105, N° 5 (09-10/2021) . - p. 231-241
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Examination of moderately halophilic bacteria in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 105, N° 5 (09-10/2021)
