[article]
| Titre : |
Characterization and evaluation of fungal enzymatic pool with unhairing activity |
| Type de document : |
texte imprimé |
| Auteurs : |
B. C. Galarza, Auteur ; M. Garro, Auteur ; J. Martegani, Auteur ; R. Hours, Auteur |
| Année de publication : |
2016 |
| Article en page(s) : |
p. 257-262 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Caractérisation
Cuirs et peaux -- Analyse
Cuirs et peaux de bovins
Enzymes fongiques
Enzymes protéolytiquesUne enzyme protéolytique est une enzyme capable de couper une protéine en plusieurs fragments ou peptides. La trypsine, la papaïne, la pepsine, la chymotrypsine, la plasmine, la subtilisine... sont capables de couper une protéine, chaque enzyme étant spécifique de certains sites particuliers de cette protéine. C'est ainsi, par exemple, qu'une immunoglobuline G est découpée par la papaïne en un fragment Fc et deux fragments Fab, comme l'a montré Porter en 1959.
Epilage enzymatique
Microscopie électronique à balayage
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
The use of enzymes in the leather industry in the beamhouse area to partially replace sulfide, can reduce to almost 50% the H2S emission. In this enzyme treatment proteolysis needs to be controlled to prevent the extension of activity to collagen. Collagen must keep its optimal conditions for the tannage process and to give high quality leather. In this sense it is necessary to characterize the enzyme from the physicochemical point of view and to study the morphological changes produced in bovine skin to control its action. An enzymatic pool of geophilic strain Trichophyton ajelloi cultured in solid media was characterized. Azocaseinolytic and keratinolytic activity was determined under different conditions. The enzymatic pool showed tolerance to high T°, optimal pH9 in bath substrates, partial inhibition in concentrations 0.1M, 0.01M and 0.001 M from metallic salts (Ca Cl2, BaCl2, KCl, CuSO4), activation by NaCl, reducing agents as Na2S03, Na2S, L-cystein hydrochloride monohydrate, thioglycolic acid and commercial anionic and non-ionic surfactants (SDS, isogràs AN, Triton, Baymol, Azymol). Inactivation exerted by. PMSF showed a serine-protease as a component of the pool (MEROPS system). When the enzymatic pool was applied for 24-48 hours under optimal conditions on bovine skin pieces, a depilatory affect was observed. The changes in the bovine skin morphology were observed by SEM, alter fixation in formaldehyde and dehydration with alcohol as part of a tentative protocol for bovine skin analysis by SEM (Scanning Electron Microscopy). |
| Note de contenu : |
- Solid-state culture
- Characterization of crude extract
- Test procedures
- SEM : sample preparation and observation |
| En ligne : |
https://drive.google.com/file/d/1Cn1wPo2dkSvZQZ62tGJGbpyJQQ1Ylr7Q/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27091 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 100, N° 5 (09-10/2016) . - p. 257-262
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