| Titre : |
New technologies for specific antiperspirant actions |
| Type de document : |
texte imprimé |
| Auteurs : |
Jessica Welzel, Auteur ; Sabine Grüdl, Auteur ; Bernhard Banowski, Auteur ; Andrea Sättler, Auteur ; Thomas Förster, Auteur ; Thomas Welss, Auteur |
| Année de publication : |
2020 |
| Article en page(s) : |
p. 46-51 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Aisselles -- Soins et hygiène
Antiperspirants
Cosmétiques
Ingrédients cosmétiques
Sels d'aluminium -- Produits de remplacement
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
Cosmetics support a well-balanced quality of life of consumers by improving their outer appearance or repressing unpleasant attributes such as inordinate sweating or smelling. Especially sweating may be socially stigmatizing when becoming excessive. However, the biological mechanisms behind perspiration are not completely understood. The only effective cosmetic antiperspirant technology to reduce sweating is based on the use of aluminum salts, which lately became publicly misperceived. Henkel Beauty Care developed a sophisticated in vitro model of the human eccrine sweat gland to screen for and identify new antiperspirant technologies substituting these aluminum salts. This proprietary cell model combines all the relevant sweat gland cells in a three-dimensional (3D) environment.
In verification tests of the cell model the in vitro simulation of both, stimulation of the sweating process and its inhibition, were shown. Further, this 3D model was used for screening of sweat-regulating actives. Thereby, we focused on the change of certain ion-levels within the cells upon treatment with potential inhibitors. Positively screened potential antiperspirant substances were subsequently tested in vivo revealing a striking in vitro / in vivo correlation.
A new biologically potent antiperspirant technology is on its way to the consumer successfully replacing the common aluminum salts. |
| Note de contenu : |
- 3D in vitro cell model of the human eccrine sweat gland
- The in vitro screening
- In vivo validation of in vitro observation
- In vitro/in vivo correlation
- Fig. 1 : Eccrine sweat gland cells in a suspension aggregate at the tip of the medium droplet in a Hanging Drop Plate forming the 3D eccrine sweat gland model
- Fig. 2 : 2D cell culture (a) and 3D eccrine sweat gland model (b) in which the cells are loaded with an ion-sensitive fluorescence dye to measure the changes of intracellular ion contents. Bar represents 100 μm
- Fig. 3 : Concentration dependent reduction of the intracellular sodium content after treatment of eccrine sweat gland cells with substance 1. ***: significantly different from 0% with p < 0.0001 (one-way ANOVA with Tukey’s post-hoc multiple comparison test). Data are mean ± SD of two independent experiments performed in sextuplet
- Fig. 4 : Photograph of a volunteer’s back in a sweat reduction study during occlusion phase
- Fig. 5 : Sweat reducing effect of test substance 1 determined on the back of volun-teers. Increasing concentrations of test substance 1 enhanced the sweat reducing potential up to the efficiency reached with conventionally used Aluminium chloro-hydrate (ACH). Data are mean of 15-20 volunteers
- Fig. 6 : Observed correlation between the intracellular sodium content determined in substance 1-treated eccrine sweat gland cells in vitro and the actual sweat reducing efficiency of substance 1 achieved on the back of volunteers in vivo |
| En ligne : |
https://drive.google.com/file/d/1uEVDnRC-MX-BGCnG11l8AZ0n5T6CuKaW/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33827 |
in SOFW JOURNAL > Vol. 146, N° 3 (03/2020) . - p. 46-51
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New technologies for specific antiperspirant actions in SOFW JOURNAL, Vol. 146, N° 3 (03/2020)
