[article]
| Titre : |
A human skin model to evaluate the protective effect of compounds against UVA damage |
| Type de document : |
texte imprimé |
| Auteurs : |
Sophie Abadie, Auteur ; Philippe Bedos, Auteur ; J. Rouquette, Auteur |
| Année de publication : |
2019 |
| Article en page(s) : |
p. 594-603 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Barrière cutanée
Dermatologie
Explant de peau
Peau -- Anatomie
Peau -- Physiologie
Rayonnement ultraviolet -- Effets physiologiques
Rayonnement ultraviolet A
Tests de sécurité
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modifications, which are responsible for both premature ageing and cancer. The aim of this study was to establish a discriminatory and sufficiently reproducible cutaneous model for evaluating UVA damage, to enable testing for effectiveness of potentially protective compounds.
- Methods : The cutaneous model is based on Human skin explants irradiated with UVA. Deleterious effects on epidermis were observed and quantified by haematoxylin–eosin staining and by immunofluorescence of y-H2Ax, cytokeratin 5, involucrin and loricrin protein. Dermis deterioration was evaluated by transmission electronic microscopy and zymography in situ.
- Results : We were able to observe and quantify deleterious effects associated with UVA irradiation: epidermal and dermal disruption, appearance of Sunburn cells, increased DNA damage and induced apoptosis. The use of this model in the evaluation of protective compounds was first confirmed using sunscreens, then further validated with a panel of active ingredients which showed beneficial effects on epidermis morphology and DNA integrity after UVA exposure.
- Conclusion : We have developed a model and a standardized protocol, based on the use of human skin explants, which allows us to explore the protective effect of active ingredients to environmental stresses such as UVA. |
| Note de contenu : |
- MATERIALS AND METHODS : Human skin tissue collection - UVA irradiation - Compounds - Histological analysis - Immunostaining - TUNEL assay - Transmission electron microscopy on dermis - Zymography in situ - Statistical analysis
- RESULTS : UVA irradiation causes epidermal disruption - UVA irradiation induces double-strand DNA breaks and apoptosis of keratinocytes - Effects of UVA irradiation on the extracellular matrix of the dermis - A cutaneous model to explore the effect of new protective compounds against UVA |
| DOI : |
10.1111/ics.12579 |
| En ligne : |
https://drive.google.com/file/d/1oEJ8PMUgmYbSCqYb-rUmWILUY8tIbO0L/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33668 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 41, N° 6 (12/2019) . - p. 594-603
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A human skin model to evaluate the protective effect of compounds against UVA damage in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 41, N° 6 (12/2019)
