A rapid quantification of hydroxyproline in leather using High-Performance Liquid Chromatography - Fluorescence Detection (HPLC-FLD) method / Priya Narayanan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVIII, N° 5 (05/2023)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVIII, N° 5 (05/2023) . - p. 183-192 Titre : A rapid quantification of hydroxyproline in leather using High-Performance Liquid Chromatography - Fluorescence Detection (HPLC-FLD) method Type de document : texte imprimé Auteurs : Priya Narayanan, Auteur ; Suresh Sethurajan, Auteur ; Mohan Vedhanayagam, Auteur ; Kalarical Janardhanan Sreeram, Auteur Année de publication : 2023 Article en page(s) : p. 183-192 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Analyse quantitative (chimie) Chromatographie en phase liquide à hautes performances Cuirs et peaux -- Analyse Cuirs et peaux -- Teneur en collagène Hydroxyproline Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : In the area of leather development research, determining collagen content through measurement of 4-Hydroxyproline (Hyp) has been major challenging task due to the interference of various leather-processing chemicals. To overcome this problem, for the first time we have used a High-Performance Liquid Chromatography coupled with Fluorescence Detection (HPLC-FLD) method for accurate determination of Hyp in leather samples through chemical derivatization of Hyp with 7-chloro 4 nitro benzofuran (NBD-Cl) reagent. The HPLC-FLD analysis was performed on a PICO TAG column with an isocratic mobile phase (80 % of 0.1M, pH 7.2 sodium acetate and 20% of acetonitrile, v/v) at a flow rate of 0.7mL/min. The detection was carried out at an excitation wavelength of 465 nm and its emission at 535nm. The retention time of Hyp was found to be ~3.5 minutes and the total run time was about 10 minutes. The method validation indicated that this analytical method is precise (3-12% RSD), accurate (90-100%), the limit of detection 0.01μg/ml, the limit of quantification - 0.03 μg/ml and linear (R2 -0.9995) over the concentration range of 0.1 -2.0 μg/ml. The obtained result indicated that the assay linear range was acceptable for repeated analysis and suitable for the complete range of hydroxyproline levels present in leather samples. Compared to the traditional method (IUC 17:1980), this analytical method demonstrates higher simplicity, specificity, reproducibility, and it could be useful for certifying leather products as well as inspecting international trade in leather and hides. Note de contenu : - EXPERIMENTAL : Materials and methods : Chemical - Chromatographic conditions - Preparation of standard hydroxyproline solution - Preparation of salple derivatization - Method validation - Linearity and concentration range - Limit of detection and quantification - Accuracy - Precision - Robustness - Estimation of fibrous protein in the tanned leather sample - RESULTS AND DISCUSSION : Method development and optimization - METHOD VALIDATION : Linearity - LOD and LOQ - ACCURACY - PRECISION : Robustness - Tanned leather sample - Table 1 : Optimization of chromatographic method through changing the various parameters - Table 2 : Summary of parameters in method validation - Table 3 : Limits of detection (LOD) and quantitation (LOQ) of proposed method - Table 4 : Precision of the proposed method - Table 5 : Accuracy of the proposed method - Table 6 : Robustness of the proposed method - Table 7 : Total collagen content calculated using the equation DOI : https://doi.org/10.34314/jalca.v118i5.7359 En ligne : https://drive.google.com/file/d/1-2G5tcy3I72WO5k_-iNVP872ZwDTwX5_/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39303 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
23954	-	Périodique	Bibliothèque principale	Documentaires	Disponible

Value added leather auxiliaries from paper and pulp industry waste / Mohan Vedhanayagam in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CX, N° 9 (09/2015)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CX, N° 9 (09/2015) . - p. 295-301 Titre : Value added leather auxiliaries from paper and pulp industry waste Type de document : texte imprimé Auteurs : Mohan Vedhanayagam, Auteur ; Tewodros Kassa, Auteur ; Kalarical Janardhanan Sreeram, Auteur ; Jonnalagadda Raghava Rao, Auteur ; Balachandran Unni Nair, Auteur Année de publication : 2015 Article en page(s) : p. 295-301 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Agents de tannage Extraction par solvant Formaldéhyde Liqueur noire -- Recyclage La liqueur noire est la liqueur de cuisson issue de la fabrication du papier kraft. Il s'agit d'une solution aqueuse composée des résidus de lignine et d'hémicellulose dissous de la pâte à papier, ainsi que des autres composés chimiques inorganiques utilisés dans le processus de dissolution. Elle contient en outre plus de la moitié de la teneur énergétique du bois amené dans le lessiveur. Normalement concentrée jusqu'à 65 % - 80 % par un évaporateur multi-effets, elle est ensuite brûlée à 1 200°C dans une chaudière de récupération afin de produire de l'énergie et récupérer les produits chimiques de cuisson. Papèterie -- Déchets -- Recyclage Récupération (Déchets, etc.) Sulfonation Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The present work involves the preparation of a retanning agent from the organics present in the black liquor generated by paper and pulp industry. Black liquor organics was extracted by using solvent extraction method and subsequently separated as acidic, non-acidic and organic compounds that were not degraded. Acidic and non acidic organics were sulfonated and further condensed with formaldehyde to obtain a product ideal for application. Sulfonation – condensation reactions were modulated to achieve particle size on par with that of commercial syntans. Condensed products from both acidic and non-acidic components were used in lieu of synthetic tanning agents in retanning. The final leathers exhibit offwhite color with good mechanical strength as compared to leathers from commercial phenolic syntan. This work reveals that the black liquor, which is a by-product of paper and pulp industry could through an innovative process, be turned into a retanning agent for leather processing. The product has the advantage of being able to replace phenol – a product with high market fluctuation. Note de contenu : - EXPERIMENTAL : Materials and methods - Extraction of acidic and non-acidic organics from black liquor - Preparation of syntan from non-acidic organics of black liquor (Syntan A) - Preparation of syntan from acidic organics (Syntan B) - FT-IR spectroscopy - Dynamic light scattering (DLS) - Performance evaluation on leather - Determination of color difference of crust leathers - Physical testing of crust leathers - Evaluation of organoleptic properties - Optical microscopic analysis - Formaldehyde content in the leather - Gas-permeability analysis - RESULTS AND DISCUSSION : Extraction of degraded lignin - FT-IR spectrum of organic and non-acidic organic components - Particle size of condensed product - Color measurements - Performance evaluation of developed syntans. 1. Evaluation of organoleptic properties - Evaluation of crust leather - II. Gas permeability analysis - Overall considerations En ligne : https://drive.google.com/file/d/1wry6s1S2NwzB3pqkvelQnrvmrMVgS7Ex/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24597 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
17423	-	Périodique	Bibliothèque principale	Documentaires	Disponible

---

1 (1 - 2 / 2)