| Titre : |
Elucidation of probable mechanism for biocidal resistance in skin-borne Bacillus Subtilis |
| Type de document : |
texte imprimé |
| Auteurs : |
S. Kavitha, Auteur ; B. Swarnakumari, Auteur ; Chandra Babu Narasimhan Kannan, Auteur ; M. Vahini, Auteur ; C. Vandhana, Auteur |
| Année de publication : |
2014 |
| Article en page(s) : |
p. 418-424 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Antimicrobiens
Bacillus subtilis
Cuirs et peaux
Résistance aux antimicrobiens
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
An attempt has been made in the present investigation to determine the probable mechanism through which a skin-borne bacterium Bacillus subtilis might develop resistance against a dithiocarbamate based biocide. Changes occurring in the cell wall constituents and morphology in the cells grown at sub-optimal level concentrations of biocide were studied based on which the probable mechanism of buildup of biocidal resistance has been proposed. There has been considerable change/reduction in cell wall constituents, peptidoglycan, diaminopimelic acid and teichoic acid due to the presence of biocides. By propidium iodide staining technique, the cell wall permeability has also been found to be reduced. The morphology of the cells as studied using scanning electron microscopy reveals that there is a shortening of rod shape of the cells due to the action of the biocide. Based on the results of the study, it is proposed that the skin-borne Bacillus subtilis encountered in leather processing might develop resistance through changes in the cell wall constituents and reduction in the cell wall permeability. |
| Note de contenu : |
- MATERIALS AND METHODS : Organism - Biocide - Determination of MIC - Growth study - Preparation of cell wall - determination of PG content - Determination of teichoic acid - Determination of DAP content - Propidium iodide staining - Scanning electron microscope (SEM) analysis
- RESULTS ANS DISCUSSION : Growth study - Cell wall analysis - Propidium iodide staining - Scanning electron microscopic (SEM) analysis |
| En ligne : |
https://drive.google.com/file/d/1WZs0BeL6WK9VvBJ6bnZzE2tLJoq6IPli/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=22499 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIX, N° 12 (12/2014) . - p. 418-424
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