| Titre : |
Dyes derived from benzo[a]phenoxazine – synthesis, spectroscopic properties, and potential application as sensors for l-cysteine |
| Type de document : |
texte imprimé |
| Auteurs : |
Aleksandra Grzelkowska, Auteur ; Jolanta Kolinska, Auteur ; Malgorzata Zaklos‐Szyda, Auteur ; Radoslaw Michalski, Auteur ; Jolanta Sokolowska, Auteur |
| Année de publication : |
2017 |
| Article en page(s) : |
p. 145-157 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Chromatographie en phase liquide à hautes performances
Colorants -- Synthèse
Etude in vitro
Photochimie
Spectrométrie de masse
Thiols
Toxicologie cellulaire
|
| Index. décimale : |
667.2 Colorants et pigments |
| Résumé : |
Several benzo[a]phenoxazine dyes containing a maleimide moiety have been synthesised and characterised by proton nuclear magnetic resonance spectroscopy and mass spectrometry. The spectroscopic properties, such as absorption and emission spectra, of these dyes – 9-maleimido-5H-benzo[a]phenoxazin-5-one (7a), 6-chloro-9-maleimido-5H-benzo[a]phenoxazin-5-one (7b), and 6-bromo-9-maleimido-5H-benzo[a]phenoxazin-5-one (7c) – were examined. These compounds were evaluated as potential fluorescent and colorimetric probes for the detection of biothiols. Results show that the studied compounds exhibit colorimetric and fluorescent responses to l-cysteine at pH 7.4. In the presence of l-cysteine, the fluorescence intensity of synthesised dyes is greatly enhanced. The addition of l-cysteine to solutions of dyes 7a to 7c also results in red shifts of their absorption bands. The cytotoxic effect of tested dyes 7a to 7c against human neuroblastoma cells (SH-SY5Y) was determined by MTT assay. The fluorescent response of these compounds to thiols SH-SY5Y was examined. The results show that the tested compounds exhibit fluorescent response in this cell line, and the benzo[a]phenoxazine dyes substituted in the 6-position with a halogen (7b and 7c) are less toxic against cells under experimental conditions than their unsubstituted analogue (7a). |
| Note de contenu : |
- EXPERIMENTAL : General - Synthesis - Photochemical experiments - Studies of the spectroscopic response of the dyes to thiols - UPLC/MS - Determination of cell viability and studies of the response to thiols in living cells
- RESULTS AND DISCUSSION : Synthesis and spectroscopic studies - Studies of the spectroscopic response to thiols - UPLC and mass spectral analyses of products formed in the reaction with L-Cys - Cell viability and response to thiols in vitro |
| DOI : |
10.1111/cote.12261 |
| En ligne : |
https://drive.google.com/file/d/15o-TDla3f6bzE6z4zT7QuFvXlTiic_rz/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=28228 |
in COLORATION TECHNOLOGY > Vol. 133, N° 2 (04/2017) . - p. 145-157
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Dyes derived from benzo[a]phenoxazine – synthesis, spectroscopic properties, and potential application as sensors for l-cysteine in COLORATION TECHNOLOGY, Vol. 133, N° 2 (04/2017)
